{"product_id":"abcam-ab62015","title":"Abcam, ab62015, Recombinant human M-CSF protein (Active)","description":"\u003cp\u003eSize: 10µg \/ 100µg \/ 500µg\u003cbr\u003e\nRecombinant human M-CSF protein (Active) is a Human Fragment protein, in the 33 to 190 aa range, expressed in Escherichia coli, with \u0026gt;95%, \u0026lt;1 EU\/µg endotoxin level, suitable for FuncS, SDS-PAGE.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;95% SDS-PAGE,\u003cbr\u003e\nEndotoxin level:\u0026lt;1 EU\/µg,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:Tag free,\u003cbr\u003e\nApplications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Method of Determination: NFS-60 cell proliferationAcceptance Criteria: ED50 ≤10 ng\/mL(≥ 1.0 x 10^5 units\/mg)The specific activity of Human M-CSF is 1.0 x 10^5 IU\/μg, which is calibrated against recombinant human M-CSF WHO International Standard.,\u003cbr\u003e\nAccession:P09603,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nReconstitution:Reconstitute at 0.1 mg\/mL in water,\u003cbr\u003e\nStorage buffer:Constituents: 0.58% Sodium chloride, 0.16% Sodium phosphate\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon reconstitution add a carrier protein (0.1% BSA), Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nM-CSF or macrophage colony-stimulating factor is a cytokine involved in the regulation of macrophage production. Alternative names for M-CSF include CSF-1 and colony-stimulating factor 1. The M-CSF protein has a molecular mass of approximately 18 to 22 kDa. M-CSF is mainly expressed in various tissues including the placenta kidney and bone marrow. It functions as a homodimer important for the survival proliferation and differentiation of mononuclear phagocyte lineage cells.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nM-CSF influences the production differentiation and function of macrophages and osteoclasts. It is not part of a large complex but interacts with its receptor the CSF1R (colony-stimulating factor 1 receptor) on the surface of target cells. This interaction promotes the survival and proliferation of the target cells playing significant roles in immune response and bone homeostasis by regulating osteoclast development.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe interaction of M-CSF with its receptor is central to several biological pathways notably the immune system pathway and bone resorption pathway. Within these pathways the binding of M-CSF to CSF1R activates downstream signaling cascades such as the PI3K\/AKT and MAPK pathways importantly affecting cell survival and differentiation. The M-CSF pathway intersects with other cytokines and factors like GM-CSF (granulocyte-macrophage colony-stimulating factor) which also regulate immune cell dynamics.\u003cbr\u003e\nDysregulation of M-CSF levels is implicated in conditions such as osteoporosis and certain cancers. In osteoporosis M-CSF’s role in osteoclast development links to increased bone resorption leading to bone loss. In cancers M-CSF overexpression may facilitate tumor-associated macrophage infiltration therefore supporting tumor progression. The interaction with CSF1R is significant as it serves as a potential target for therapeutic strategies aimed at modulating macrophage activity in these diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843959017641,"sku":"ab62015","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab62015","provider":"Iright","version":"1.0","type":"link"}