{"product_id":"abcam-ab62292","title":"Abcam, ab62292, Recombinant Raf1 (mutated Y340E + Y341E) protein (Active)","description":"\u003cp\u003eSize: 5µg \/ 10µg\u003cbr\u003e\nRecombinant Raf1 (mutated Y340E + Y341E) protein (Active) is a Human Fragment protein, in the 306 to 648 aa range, expressed in Baculovirus infected Sf9 cells, with \u0026gt;80%, suitable for FuncS, WB.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;80% SDS-PAGE,\u003cbr\u003e\nExpression system:Baculovirus infected Sf9 cells,\u003cbr\u003e\nTags:GST tag N-Terminus,\u003cbr\u003e\nApplications:FuncS, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:The specific activity of RAF1 was determined to be ~6,000 nmol\/min\/mg in a coupled assay as per antibody assay protocol.,\u003cbr\u003e\nAccession:P04049,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nStorage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0029% EDTA, 0.0017% PMSF\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRaf1 also known as c-Raf or Raf-1 is a serine\/threonine-protein kinase that plays an important role in cell division differentiation and survival. Its molecular weight is approximately 74 kDa. Raf1 is part of the MAPK\/ERK signaling pathway and is ubiquitously expressed in many tissues including the brain heart and liver. As a component of the signaling cascade its activation translates extracellular signals into intracellular responses.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRaf1 regulates important cellular processes by activating downstream kinases in response to external stimuli. Raf1 forms a complex with other proteins such as Ras facilitating its role as an essential component of the MAPK\/ERK pathway. Upon activation by Ras Raf1 phosphorylates and activates MEK1 and MEK2 which in turn activate the ERK1 and ERK2. This signaling axis is involved in controlling gene expression and cellular proliferation.\u003cbr\u003e\nPathways\u003cbr\u003e\nRaf1 is integrally involved in the MAPK\/ERK pathway which is critical for transducing signals from growth factors and mitogens. It relates closely with proteins such as Ras MEK and ERK in this pathway. The pathway is important for regulating cellular responses to various stimuli and is particularly involved in processes such as cell cycle control and apoptosis.\u003cbr\u003e\nRaf1 has associations with certain types of cancer and Noonan syndrome. In various cancers mutations or dysregulation of Raf1 or related components such as Ras can lead to uncontrolled cellular proliferation. In Noonan syndrome Raf1 mutations result in anomalies in the Ras\/MAPK pathway which can impact normal development. Raf1's interactions in these pathways indicate its relevance as a potential target for therapeutic intervention.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843902296233,"sku":"ab62292","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab62292","provider":"Iright","version":"1.0","type":"link"}