{"product_id":"abcam-ab65620","title":"Abcam, ab65620, Glycogen Assay Kit","description":"\u003cp\u003eSize: 100Test \/ 2000Test\u003cbr\u003e\nGlycogen Assay Kit ab65620 is a no-wash glycogen assay with two 30-minute incubation steps. In the assay, glucoamylase acts on glycogen, and the resulting glucose is oxidized. Readout on any colorimetric (570 nm) or fluorometric (Ex\/Em 535\/587 nm) plate reader. - Complete kit format with full assay protocol and standard curve for quantitation - Published extensively with tissue lysates (including liver and muscle) and cultured cell lysates - Cited in over 140 publications - Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric\/Fluorometric,\u003cbr\u003e\nSample types:Urine, Tissue, Cell culture supernatant, Other biological fluids,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nSensitivity:\u0026gt; 0.04 µg\/mL,\u003cbr\u003e\nRange:40 - 2000 ng\/well,\u003cbr\u003e\nAssay time:1h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nGlycogen Assay Kit ab65620 is an easy and accurate assay to measure glycogen levels in biological samples.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nIn the glycogen assay protocol, glucoamylase hydrolyzes the glycogen to glucose which is then specifically oxidized to produce a product that reacts with OxiRed probe to generate color (570 nm) and fluorescence (Ex 535\/Em 587). The assay can detect glycogen 0.04 to 2 mg\/ml.\u003cbr\u003e\nGlycogen assay protocol summary\u003cbr\u003e\n- Add samples and standards to wells\u003cbr\u003e\n- Add hydrolysis enzyme mix and incubate for 30 min\u003cbr\u003e\n- Add reaction mix and incubate for 30 min\u003cbr\u003e\n- Analyze with microplate reader\u003cbr\u003e\nRelated Glycogen assay products\u003cbr\u003e\nIf your sample is likely to contain reducing substances, we recommend using Glycogen Assay Kit II\u003cbr\u003e\nab169558\u003cbr\u003e\n, as reducing substances may interfere with the assay detection method used with ab65620.\u003cbr\u003e\nab169558\u003cbr\u003e\nuses an alternative assay method, where glucoamylase hydrolyzes glycogen to glucose, followed by an enzymatic step which produces NADH, which is then used to reduce a tetrazolium dye, producing a colored product.\u003cbr\u003e\nIf you are running a 384 well assay, we recommend Glycogen Colorimetric Assay Kit\u003cbr\u003e\nab282931\u003cbr\u003e\n, which uses an identical assay method to ab65620, and is formatted for 384 well use.\u003cbr\u003e\nHow other researchers are using Glycogen Assay Kit ab65620\u003cbr\u003e\nThis glycogen assay kit has been used in publications in a variety of sample types, including:\u003cbr\u003e\n- Human: muscle tissue\u003cbr\u003e\n- Mouse: muscle tissue lysates\u003cbr\u003e\n, muscle and liver tissue\u003cbr\u003e\n, liver\u003cbr\u003e\n, cultured muscle myotubes\u003cbr\u003e\n, astrocyte primary cell lysates\u003cbr\u003e\n- Rat: liver\u003cbr\u003e\n, neuron-astrocyte co-cultures\u003cbr\u003e\n- Bacteria: M. buryatense\u003cbr\u003e\n, Haemophilus influenzae\u003cbr\u003e\nReferences: 1 - Vaughan D et al 2016, Trewin AJ et al 2015; 2 - Baligand C et al 2017, Riedl et al 2016, Wicks SE et al 2015, Todd AG et al 2015, Lundell LS et al 2019, Kim HY et al 2016, Amoasii et al 2016; 3 - Xirouchaki CE et al 2016, Pamir N et al 2015, Zachwieja NJ et al 2016; 4 - Pursell et al 2018; 5 - Park M et al 2016; 6 - Choudhury GR et al 2015; 7 - Xiang L et al 2014, Guo J et al 2018; 8 - Sobieski C et al 2018; 9 - Puri AW et al 2015; 10 - Wu S et al 2014\u003cbr\u003e\nGlycogen assay methods\u003cbr\u003e\nThere are 3 major methods used to assay glycogen levels:\u003cbr\u003e\n- Use of enzymes to produce glucose or glucose-1-phosphate from glycogen, with production of a colored reaction product (Passonneau et al 1974 PMID 4844560), such as in ab65620.\u003cbr\u003e\n- The anthrone method (Roe JH et al 1966, PMID 4289896) which uses alkaline digestion of glycogen, precipitation of undigested proteins with excess acid, and adds the anthrone reagent to detect glucose in a spectrophotomer. The major disadvantage of the anthrone method is the use of hazardous concentrated sulfuric acid which is heated in a \u0026gt;90°C water bath.\u003cbr\u003e\n- The phenol‐sulfuric acid method (described in Schaubroeck et al 2022, PMID 35179318), in which sulfuric acid dehydrates glycogen to 5‐hydroxymethylfurfural, which reacts with phenol to generate an orange‐colored solution, the absorbance of which can be measured spectrophotometrically. The major disadvantages of this method are the requirement to handle and resuspend pellets after centrifugation which can be fiddly, and the use of hazardous concentrated sulfuric acid and phenol.\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nLearn more about our\u003cbr\u003e\ntools for obesity research\u003cbr\u003e\n, including antibodies and ELISA kits to adipogenesis and lipid metabolism markers, tools for GLP-1 receptor agonist research, and enzymatic assays to key biochemicals and enzymes involved in obesity-related metabolism.\u003cbr\u003e\nOther notes\u003cbr\u003e\nThis product was previously called K646 BioVision Glycogen Colorimetric\/Fluorometric Assay Kit. Abcam acquired BioVision in 2021.\"\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGlycogen is a large branched polymer of glucose serving as a form of energy storage in animals and fungi. It is sometimes called animal starch. The molecular mass of glycogen varies but it's typically around 1000000 Da. Glycogen is stored mainly in the liver and muscles. In the liver it acts as a glucose reservoir for the body while in muscles it provides energy during physical activity. In glycogen studies the use of glycogen assays glycogen measurement techniques and glycogen kits is common for quantifying its levels.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGlycogen plays an important role in energy homeostasis by storing glucose that can be rapidly mobilized. Glycogen synthase and glycogen phosphorylase are critical enzymes that synthesize and degrade glycogen respectively. Glycogen synthesis and degradation are tightly regulated processes that occur in response to hormonal signals like insulin and glucagon. Glycogen often interacts with proteins that are part of complexes regulating its breakdown ensuring glucose availability when needed by the cells. Scientists often use glycogen assay kits to measure glycogen levels and the effects of its hydrolysis.\u003cbr\u003e\nPathways\u003cbr\u003e\nGlycogen metabolism fits into key metabolic processes such as glycolysis and gluconeogenesis. In glycolysis the breakdown of glycogen provides glucose-1-phosphate which can further undergo conversion to glucose-6-phosphate entering the glycolytic pathway. Glycogen is also related to glycogenin a protein essential for glycogen synthesis acting as a primer for chain elongation. Within gluconeogenesis glycogen contributes glucose units for the formation of new glucose molecules maintaining blood sugar levels during fasting conditions.\u003cbr\u003e\nGlycogen storage diseases and type 2 diabetes involve glycogen-related dysfunctions. Glycogen storage diseases are a group of metabolic disorders related to defects in glycogen synthase and glycogen phosphorylase leading to abnormal glycogen accumulation or structure. Type 2 diabetes is associated with impaired glycogen synthesis and breakdown often linked to insulin resistance. Understanding the role of glycogen in these conditions helps uncover therapeutic targets with studies utilizing glycogen tests and specific assays like those involving k648.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843596538025,"sku":"ab65620","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab65620","provider":"Iright","version":"1.0","type":"link"}