{"product_id":"abcam-ab82104","title":"Abcam, ab82104, Recombinant human TIMP1 protein","description":"\u003cp\u003eSize: 10µg\u003cbr\u003e\nRecombinant human TIMP1 protein is a Human Full Length protein, expressed in Escherichia coli, with \u0026gt;95%, suitable for SDS-PAGE, WB, FuncS.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;95% SDS-PAGE,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:Tag free,\u003cbr\u003e\nApplications:SDS-PAGE, WB, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Biological Activity : TIMP1 activity was measured by its ability to inhibit human MMP1 induced hydrolysis of a chromogenic peptide substrate at room temperature. Half maximal inhibition was obtained at a TIMP1 concentration of approximately 0.5 μg\/ml, when using an MMP1 concentration of 1.6 μg\/ml.,\u003cbr\u003e\nAccession:P01033,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nReconstitution:Reconstitute in water\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg\/ml. Do not vortex (can be stored at 4C for up to 1 week).\u003cbr\u003e\nFor extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20C to -80C.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage duration-A few weeks, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTissue Inhibitor of Metalloproteinase 1 (TIMP1) is a protein with a molecular mass of approximately 28 kDa. The protein inhibits metalloproteinases by binding to them preventing the breakdown of the extracellular matrix. This action regulates extracellular environment and tissue remodeling. TIMP1 is also known by the name Erythroid-Potentiating Activity (EPA). It is expressed in various tissues including the liver and the brain and can be found in high levels in the blood serum.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTIMP1 plays several essential roles beyond inhibiting metalloproteinases. It supports cell proliferation and influences apoptosis. It is part of a larger TIMP family and does not function as part of a complex per se but interacts closely with matrix metalloproteinases (MMPs). By regulating MMP activity TIMP1 balances processes like tissue growth and inflammatory responses which are important for normal development and repair.\u003cbr\u003e\nPathways\u003cbr\u003e\nTIMP1 participates in the regulation of the matrix metalloproteinase pathway impacting tissue homeostasis and repair. It closely associates with proteins such as MMP-9 and MMP-2 within this pathway. TIMP1 also plays a role in cytokine signaling pathways which influence immune responses by interacting with other signaling molecules and receptors that govern these pathways.\u003cbr\u003e\nTIMP1's regulation of MMPs links it to cancer and cardiovascular disease progression. Overexpression of TIMP1 associates with tumor growth and metastasis where it can modulate interactions with other proteins like MMP-9 leading to altered tissue invasion and angiogenesis. Additionally TIMP1 imbalance connects to tissue fibrosis in cardiovascular disorders working in tandem with proteins such as MMP-2 which affects the structural remodeling and function of tissues within the cardiovascular system.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843801043113,"sku":"ab82104","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab82104","provider":"Iright","version":"1.0","type":"link"}