{"product_id":"abcam-ab8211","title":"Abcam, ab8211, FITC Anti-alpha smooth muscle Actin antibody [1A4]","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nAnti-alpha smooth muscle Actin antibody [1A4] - FITC conjugated (ab8211) is a mouse monoclonal antibody detecting alpha smooth muscle Actin in  Western Blot, ICC\/IF . Suitable for  Human . - KO validated for confirmed specificity - Over 70 publications - Trusted since 2001\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:1A4,\u003cbr\u003e\nIsotype:IgG2a,\u003cbr\u003e\nLight chain type:kappa,\u003cbr\u003e\nConjugation:FITC,\u003cbr\u003e\nExcitation\/Emission:Ex: 495nm, Em: 519nm,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nFITC Anti-alpha smooth muscle Actin antibody [1A4] (ab8211) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human samples.\u003cbr\u003e\nWhat is the molecular weight of alpha smooth muscle Actin?\u003cbr\u003e\nAnti-alpha smooth muscle Actin [1A4] FITC (ab8211) specifically detects a band for alpha smooth muscle Actin (UniProt: P62736) at a molecular weight of 42kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-alpha smooth muscle Actin [1A4] FITC (ab8211) was first used in a scientific publication in 2001 and has been cited over 70 times in peer-reviewed journals.\u003cbr\u003e\nReviewed by scientists\u003cbr\u003e\nAnti-alpha smooth muscle Actin [1A4] FITC (ab8211) has over 5 independent reviews from customers.\u003cbr\u003e\nRelated products\u003cbr\u003e\nAntibody clone 1A4 is also available pre-conjugated to a variety of labels for your convenience – Anti-alpha smooth muscle Actin FITC [1A4] (ab8211).\u003cbr\u003e\nSpecificity confirmed\u003cbr\u003e\nThe specificity of FITC Anti-alpha smooth muscle Actin antibody [1A4] (ab8211) has been confirmed by Immunocytochemistry testing in ACTA2 Knockout HeLa cells.\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [1A4] also available for your convenience:\u003cbr\u003e\nab7817\u003cbr\u003e\n, FITC - ab8211, Biotin -\u003cbr\u003e\nab125057\u003cbr\u003e\n, Alexa Fluor® 488 -\u003cbr\u003e\nab184675\u003cbr\u003e\n, Alexa Fluor® 594 -\u003cbr\u003e\nab202368\u003cbr\u003e\n, HRP -\u003cbr\u003e\nab203696\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab240654\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Avoid freeze \/ thaw cycle, Store in the dark\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAlpha smooth muscle actin (α-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of α-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of α-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAlpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.\u003cbr\u003e\nPathways\u003cbr\u003e\nAlpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA\/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.\u003cbr\u003e\nAlpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of α-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of α-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of α-SMA in disease development.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844272312489,"sku":"ab8211","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab8211","provider":"Iright","version":"1.0","type":"link"}