{"product_id":"abcam-ab8274","title":"Abcam, ab8274, Anti-Salmonella typhimurium LPS antibody [1E6]","description":"\u003cp\u003eSize: 250µg\u003cbr\u003e\nAnti-Salmonella typhimurium LPS antibody [1E6] (ab8274) is a mouse monoclonal antibody detecting Salmonella typhimurium LPS in  Western Blot, Flow Cytometry, ICC\/IF, ELISA, EM, Dot Blot . Suitable for  Salmonella typhimurium . - Over 20 publications - Trusted since 2002\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:1E6,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nLight chain type:unknown,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Salmonella typhimurium,\u003cbr\u003e\nApplications:EM, ICC\/IF, ELISA, Flow Cyt, Dot, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nSpecificity:This antibody is directed against lipopolisaccharides of Salmonella typhimurium\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-Salmonella typhimurium LPS antibody [1E6] (ab8274) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunocytochemistry\/immunofluorescence (ICC\/IF), ELISA, Electron microscopy (EM), Dot Blot in Salmonella typhimurium samples.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-Salmonella typhimurium LPS [1E6] (ab8274) was first used in a scientific publication in 2002 and has been cited over 20 times in peer-reviewed journals.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.1% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSalmonella typhimurium LPS also known as lipopolysaccharide forms a vital component of the outer membrane of Gram-negative bacteria such as Salmonella. This complex molecule consists of a lipid A domain a core oligosaccharide and an O-antigen polysaccharide chain. The lipid A component with an estimated mass of roughly 10 kDa is the main contributor to LPS endotoxin activity. In Salmonella the LPS is expressed in the bacterial cell wall providing structural integrity and defense against environmental stresses. Its composition varies among different Salmonella strains influencing the bacterium's virulence and interaction with host immune systems.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSalmonella typhimurium LPS engages in stimulating immune responses. An important part of the LPS structure the lipid A moiety activates the innate immune system by binding to Toll-like receptor 4 (TLR4) on host immune cells. This interaction triggers a cascade of pro-inflammatory cytokine production. As part of the outer membrane of Salmonella LPS works in synergy with other membrane proteins to form the protective barrier for the bacteria. The variability in the O-antigen portion aids in evasion of host immune responses allowing different Salmonella serotypes to survive and cause infection.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe response of TLR4 to Salmonella typhimurium LPS forms a central axis in innate immune signaling. This pathway activates NF-kB a transcription factor pivotal in inducing cytokine genes thereby intensifying the antibacterial response. LPS interactions not only involve TLR4 but also influence pathways such as the MAPK signaling cascade further regulating immune responses. These pathways ensure rapid immune mobilization to control bacterial infections highlighting the significant role LPS plays in pathogen-host interactions.\u003cbr\u003e\nSalmonella typhimurium LPS remains an important factor in the pathogenesis of salmonellosis and septic shock. LPS as a potent endotoxin contributes to systemic inflammation during septicemia by over-activating the immune system. Its interaction with TLR4 leads to high levels of inflammatory cytokines resulting in symptoms associated with both intestinal and systemic infections. The molecular attributes of LPS particularly lipid A connect to disease outcomes and also offer potential targets for therapeutic intervention in controlling severe inflammatory responses during Salmonella infections.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855703560361,"sku":"ab8274","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab8274","provider":"Iright","version":"1.0","type":"link"}