{"product_id":"abcam-ab83367","title":"Abcam, ab83367, Acid Phosphatase Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 500Test\u003cbr\u003e\nAcid Phosphatase Assay Kit ab83367 is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure acid phosphatase activity in serum and other samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate,\u003cbr\u003e\nAssay type:Enzyme activity,\u003cbr\u003e\nSensitivity:\u0026gt; 0.001 mM,\u003cbr\u003e\nRange:0.001 - 1 mM,\u003cbr\u003e\nAssay time:1h 10m,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAcid Phosphatase Assay Kit ab83367 is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure acid phosphatase activity in serum and other samples.\u003cbr\u003e\nThe acid phosphatase assay protocol uses\u003cbr\u003e\n-nitrophenyl phosphate (\u003cbr\u003e\nNPP) as a phosphatase substrate which turns yellow (absorbance max 405 nm) when dephosphorylated by acid phosphatase.\u003cbr\u003e\nAcid phosphatase assay protocol summary:\u003cbr\u003e\n- add samples and standards to wells\u003cbr\u003e\nNPP to samples\u003cbr\u003e\n- add acid phosphatase enzyme to standards\u003cbr\u003e\n- incubate for 60 min\u003cbr\u003e\n- add stop solution\u003cbr\u003e\n- analyze with microplate reader\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K411 Acid Phosphatase Activity Colorimetric Assay Kit. K411-500 is the same size as the 500 test size of ab83367.\u003cbr\u003e\nAcid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease.\u003cbr\u003e\npNPP Assays\u003cbr\u003e\npNPP assays are a class of phosphatase assays using p-nitrophenyl phosphate (pNPP) as a phosphatase substrate. They can be used to measure alkaline phosphatase, neutral phosphatase, and acid phosphatase activity. These different types of phosphatases are active respectively in alkaline, neutral, and acid assay buffers. pNPP assays include Alkaline Phosphatase Assay Kit\u003cbr\u003e\nab83369\u003cbr\u003e\nand Acid Phosphatase Assay Kit ab83367.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAcid phosphatase also known as tartrate-resistant acid phosphatase (TRAP) or prostatic acid phosphatase (PAP) is an enzyme that catalyzes the hydrolysis of phosphate esters in an acidic environment. It has a molecular mass of approximately 50 kDa and is expressed in various human tissues including the prostate bone liver and spleen. Within the prostate it breaks down and releases phosphate from different compounds and it plays a role in bone resorption by osteoclasts.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis enzyme contributes to multiple cellular processes including the regulation of bone metabolism and prostate health. Acid phosphatase when acting as TRAP is often found within osteoclast lysosomes where it degrades bone matrix components. It does not form part of a complex but functions independently to modulate these processes. Prostatic acid phosphatase in seminal fluid has additional roles particularly in prostate tissue affecting cellular proliferation and motility.\u003cbr\u003e\nPathways\u003cbr\u003e\nAcid phosphatase participates in both bone resorption and lysosomal degradation pathways. In bone metabolism it works alongside proteins such as cathepsin K assisting in the breakdown of matrix constituents during bone remodeling. In lysosomal pathways it complements activities of enzymes like lysosomal acid lipase helping in recycling cellular materials. These interactions underline its involvement in maintaining cellular homeostasis through these critical biological pathways.\u003cbr\u003e\nAcid phosphatase relates significantly to prostate cancer and bone disorders like osteoporosis. Elevated levels of prostatic acid phosphatase in serum serve as a marker for prostate cancer linking it to androgen receptor pathways critical in disease progression. In bone disorders TRAP's role in osteoclast function connects it to conditions such as osteoporosis where its interaction with integrin receptor influences bone density regulation. Understanding these connections can aid in developing diagnostic and therapeutic strategies for these diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843618689193,"sku":"ab83367","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab83367","provider":"Iright","version":"1.0","type":"link"}