{"product_id":"abcam-ab93678","title":"Abcam, ab93678, Antigen Retrieval Buffer (100X Citrate Buffer)","description":"\u003cp\u003eSize: 125mL \/ 250mL\u003cbr\u003e\nAntigen retrieval (100X citrate buffer) (ab93678) is used in heat-induced antigen retrieval (HIER) to enhance staining on formalin-fixed, paraffin-embedded tissue sections with our primary antibody range during IHC. Part of our Lab essentials imaging workflow offering. - Provided in 100X solution (pH 5.1-5.5) for easy dilution preparation - Optimized for citrate buffer-treated antibodies - Available in different sizes to fit your experimental needs - Proven performance: cited in over 120 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nApplications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nStorage buffer:pH: 5.1 - 5.5Constituents: Citrate Buffer, 1.5% 2-butoxyethanol, 1% Hydrochloric acid\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAntigen Retrieval Buffer (100X Citrate Buffer) is a special solution that enables rehydration and antigen retrieval to be performed in formalin-fixed, paraffin-embedded tissue sections with one step. It is optimal for use with primary antibodies that require citrate buffer pretreatment.\u003cbr\u003e\nThis product contains detergent for emulsification of the paraffin.\u003cbr\u003e\n1X Dilution:\u003cbr\u003e\nThe 100X stock solution should be diluted 100-fold with distilled water before use.\u003cbr\u003e\nProtocol:\u003cbr\u003e\n1. Place paraffin-embedded slides in 1x Antigen Retrieval Buffer; cover with a vented plastic wrap, place in microwave and set high power to boil and then set low power to keep it boiling for 10 min. Let the sections cool in the microwave for at least 20 min.\u003cbr\u003e\n2. Wash sections with hot tap water for 1 min.\u003cbr\u003e\n3. Wash sections in buffer for 2 x 3 minutes.\u003cbr\u003e\n4. Continue with IHC protocol as normal.\u003cbr\u003e\nOther kits and reagents for IHC\u003cbr\u003e\nOther antigen retrieval buffers include:\u003cbr\u003e\nTris-EDTA buffer pH 9.0 ab93684\u003cbr\u003e\nEDTA buffer pH 8.0 ab93680\u003cbr\u003e\nTris buffer pH 10.0 ab93682\u003cbr\u003e\n, or see the full list of\u003cbr\u003e\nantigen retrieval buffers and enzymes\u003cbr\u003e\nFind more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the\u003cbr\u003e\nIHC kits and reagents guide\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847571001513,"sku":"ab93678","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab93678","provider":"Iright","version":"1.0","type":"link"}