{"product_id":"abcam-ab94136","title":"Abcam, ab94136, XPD overexpression 293T lysate (whole cell)","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nXPD overexpression 293T lysate (whole cell) suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.\u003cbr\u003e\nKey facts\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nForm:LiquidSee storage information\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab94136 is a 293T cell transfected lysate in which Human XPD has been transiently over-expressed using a pCMV-XPD plasmid. The lysate is provided in 1X Sample Buffer.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nXPD also known as ERCC2 is an essential helicase enzyme belonging to the family of ATP-dependent DNA helicases. It consists of approximately 761 amino acids and has a mass of around 87 kDa. XPD functions mechanically by unwinding double-stranded DNA which is important for various DNA repair processes. This protein is expressed ubiquitously in human tissues highlighting its critical role in maintaining genomic integrity.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nXPD catalyzes the unwinding of DNA within the transcription factor IIH (TFIIH) complex. This complex facilitates DNA repair and transcription initiation. XPD participates in the nucleotide excision repair (NER) pathway where it contributes to repairing damaged DNA by excising damaged nucleotides. Its role in transcription-coupled repair and general transcription initiation underlines its importance in cellular homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nDNA repair and general transcription processes are significantly affected by XPD. It plays an integral role in nucleotide excision repair (NER) and transcription. Within these pathways XPD works closely with other proteins such as XPA which assists in damage verification and incision near DNA lesions. XPD's unwinding function is essential for the correct orientation of DNA during transcription and repair events ensuring accurate gene expression and stability.\u003cbr\u003e\nXPD mutations link to xeroderma pigmentosum (XP) and trichothiodystrophy (TTD). These conditions often result in increased sensitivity to ultraviolet (UV) radiation and various developmental abnormalities. XPD mutations impair the repair of UV-induced DNA damage causing symptoms in both XP and TTD. XPD also connects with ERCC1 a protein important for repair synthesis and DNA incision highlighting its broad impact on human health when its function is compromised.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845115400361,"sku":"ab94136","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab94136","provider":"Iright","version":"1.0","type":"link"}