{"product_id":"abcam-ab99996","title":"Abcam, ab99996, Human Cardiotrophin 1 ELISA Kit (CTF1)","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Cardiotrophin 1 ELISA Kit (CTF1) is a Sandwich (quantitative) ELISA for the measurement of Human Cardiotrophin 1 (CTF1) in Human in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 20 pg\/mL,\u003cbr\u003e\nRange:16.46 - 12000 pg\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's Cardiotrophin 1 (CTF1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an\u003cbr\u003e\nin vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay for the quantitative measurement of Human Cardiotrophin 1 in plasma and cell culture supernatants. (Human Cardiotrophin 1 concentration is pretty low in normal plasma, it may not be detected in this assay).\u003cbr\u003e\nThis assay employs an antibody specific for Human Cardiotrophin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Cardiotrophin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Cardiotrophin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Cardiotrophin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nWe have not been able to detect the endogenous Human Cardiotrophin 1 in normal serum with ab99996, only in serum spiked with Human Cardiotrophin 1.\u003cbr\u003e\nOptimization may be required with urine samples.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855625506985,"sku":"ab99996","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab99996","provider":"Iright","version":"1.0","type":"link"}