{"product_id":"biolegend-100232","title":"Biolegend, 100232, Brilliant Violet 785™ anti-mouse CD3 Antibody, 50μg","description":"\u003cp\u003eCD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α\/β or γ\/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide\/MHC antigen complex\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: γδTCR-positive T-T hybridoma D1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.\u003cbr\u003e\nConcentration: µg sizes: 0.2 mg\/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780\/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Arruda DC, et al. 2022. J Control Release. 350:228. PubMed Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed Caradonna TM, et al. 2022. Cell Rep. 41:111628. PubMed Barberio AE, et al. 2023. Bioeng Transl Med. 8:e10453. PubMed Liu J, et al. 2023. Nat Commun. 14:2806. PubMed Borbet TC, et al. 2023. iScience. 26:106810. PubMed Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed Yang H, et al. 2023. Cell Death Differ. 30:560. PubMed Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed Nanishi E, et al. 2023. NPJ Vaccines. 8:18. PubMed Yousif AS, et al. 2020. Immunity. 54(2):235-246.e5. PubMed Barberio AE, et al. 2020. ACS Nano. 14:11238. PubMed Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed Hering L, et al. 2021. Int J Mol Sci. 22:. PubMed Gingrich AA, et al. 2021. Front Immunol. 12:670309. PubMed Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed Glassman CR, et al. 2021. eLife. 10:00. PubMed Sangesland M, et al. 2020. Cell Reports. 32(8):108065. PubMed Lewis EL, et al. 2021. Front Immunol. 12:741518. PubMed Carter TJ, et al. 2021. Small. 17:e2005241. PubMed Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed Chen YG, et al. 2020. Molecular Cell. 76(1):96-109. PubMed Ronsard L, et al. 2021. Front Immunol. 12:730471. PubMed Li H, et al. 2020. Nature. 584:274. PubMed Shreeve N, et al. 2021. Immunity. 54(6):1231-1244.e4. PubMed Riding AM, et al. 2022. iScience. 25:104660. PubMed Frost JN, et al. 2021. Med (N Y). 2:164. PubMed Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed Goh W, et al. 2020. Cell Rep. 33:108285. PubMed Lofano G, et al. 2015. J Immunol. 195: 1617-1627. PubMed Rouleau N, et al. 2020. Immunohorizons. 0.695833333. PubMed Wang W, et al. 2019. Cell Rep. 28:486. PubMed Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed Montalban-Arques A, et al. 2021. Cell Host Microbe. :. PubMed Nanishi E, et al. 2022. Commun Biol. 5:790. PubMed He X, et al. 2021. J Immunother Cancer. 9:. PubMed Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed\u003cbr\u003e\nRRID: AB_11218805 (BioLegend Cat. No. 100231) AB_2562554 (BioLegend Cat. No. 100232)\u003cbr\u003e\nStructure: Ig superfamily, CD3\/TCR, 20 kD\u003cbr\u003e\nDistribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells\u003cbr\u003e\nFunction: Antigen recognition, TCR signal transduction, T cell activation\u003cbr\u003e\nLigand\/Receptor: Peptide antigen\/MHC-complex\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263.\u003cbr\u003e\nGene ID: 12502\u003cbr\u003e\nUniProt: View information about CD3 on UniProt.org\u003cbr\u003e\nClone: 17A2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T cell antigen receptor complex, T3\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860825788585,"sku":"100232","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-100232","provider":"Iright","version":"1.0","type":"link"}