{"product_id":"biolegend-100237","title":"Biolegend, 100237, Brilliant Violet 605™ anti-mouse CD3 Antibody, 125microl","description":"\u003cp\u003eCD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α\/β or γ\/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide\/MHC antigen complex\u003cbr\u003e\n125microl\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: γδTCR-positive T-T hybridoma D1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610\/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Yu‐Han Chang et al. 2017. Immunity. 47(5):943-958 . PubMed Chandran S, et al. 2020. Front Immunol. 11:787. PubMed Lin J, et al. 2022. Adv Sci (Weinh). 9:e2202633. PubMed Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed Dijkman K, et al. 2023. NPJ Vaccines. 8:66. PubMed Oliver AJ, et al. 2020. Oncoimmunology. 9:1802979. PubMed Pettini E, et al. 2021. Vaccines (Basel). 9:. PubMed Mancini M, et al. 2021. Sci Rep. 11:21171. PubMed Mills EL, et al. 2022. Cell Metab. 34:140. PubMed VanDyke D, et al. 2022. Cell Rep. 41:111478. PubMed Merk VM, et al. 2023. Genes Immun. 24:52. PubMed Dickerson KM, et al. 2022. Blood Cancer Discov. 3:240. PubMed Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed Rosenkranz SC, et al. 2021. eLife. 10:00. PubMed Thelin MA, et al. 2017. Diabetes. 66:2220. PubMed Mancini M, et al. 2019. J Immunol. 202:1479. PubMed Tata A, et al. 2021. Oncoimmunology. 10:1933808. PubMed Aguilera T, et al. 2016. Nat Commun. 7:13898. PubMed Barry KC, et al. 2018. Nat Med. 24:1178. PubMed Pokrovskii M, et al. 2019. Immunity. 51:185. PubMed Wabitsch S, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1166. PubMed Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed Stein RR, et al. 2018. Elife. 7:e30916. PubMed Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed Böttcher JP, et al. 2018. Cell. 172:1022. PubMed Israelow B, et al. 2021. Sci Immunol. 6:eabl4509. PubMed Yu X, et al. 2021. Nature. 594:560. PubMed Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed An J, et al. 2022. iScience. 25:103570. PubMed Ulrich-Lewis JT, et al. 2022. Front Immunol. 13:861710. PubMed McBride KM, et al. 2019. Front Oncol. 9:517. PubMed Palazon A, et al. 2017. Cancer Cell. . 10.1016\/j.ccell.2017.10.003. PubMed Schuldt N, et al. 2015. PLoS One. 10: 0145762. PubMed Camell CD, et al. 2020. Cell Metabolism. 30(6):1024-1039.e6.. PubMed Zhang W, et al. 2022. Nat Methods. 19:759. PubMed Breznik JA, et al. 2021. The Journal of Physiology. 599(8):2169-2195. PubMed Lee SC et al. 2018. Cancer cell. 34(2):225-241 . PubMed Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed Rosser EC, et al. 2020. Cell Metabolism. 31(4):837-851. PubMed Ferrer-Font L, et al. 2020. Elife. 9:. PubMed Voigt A, et al. 2015. Biol Open. 10.1242\/bio.013771. PubMed Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed Ryu S, et al. 2021. eLife. 0.416666666666667. PubMed Aguilera TA, et al. 2020. Clin Cancer Res. 26:2972. PubMed Alam IS, et al. 2020. Cancer Res. 80:4780. PubMed Lee CH, et al. 2018. Elife. 7:e32532. PubMed Ito Y, et al. 2021. Cell Reports. 35(4):109052. PubMed Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed Matei DE, et al. 2021. Med (N Y). 2:864. PubMed\u003cbr\u003e\nRRID: AB_2562039 (BioLegend Cat. No. 100237)\u003cbr\u003e\nStructure: Ig superfamily, CD3\/TCR, 20 kD\u003cbr\u003e\nDistribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells\u003cbr\u003e\nFunction: Antigen recognition, TCR signal transduction, T cell activation\u003cbr\u003e\nLigand\/Receptor: Peptide antigen\/MHC-complex\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263.\u003cbr\u003e\nGene ID: 12502\u003cbr\u003e\nUniProt: View information about CD3 on UniProt.org\u003cbr\u003e\nClone: 17A2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T cell antigen receptor complex, T3\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860825624745,"sku":"100237","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-100237","provider":"Iright","version":"1.0","type":"link"}