{"product_id":"biolegend-100244","title":"Biolegend, 100244, Biotin anti-mouse CD3 Antibody, 500μg","description":"\u003cp\u003eCD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α\/β or γ\/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide\/MHC antigen complex\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: γδTCR-positive T-T hybridoma D1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested IHC-F - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed Gargaro M, et al. 2022. Immunity. 55:1032. PubMed Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed Slusarczyk P, et al. 2023. Elife. 12: . PubMed Lopez R, et al. 2022. Nat Biotechnol. 40:1360. PubMed Brian BF, et al. 2022. Sci Adv. 8:eabj5227. PubMed Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed Thierry GR, et al. 2018. J Exp Med. 215:2972. PubMed Shao B, et al. 2018. Mol Med Rep. 18:920. PubMed Barry KC, et al. 2018. Nat Med. 24:1178. PubMed Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed Joachim R, Suber F, and Kobzik L 2017. Sci Rep. . 10.1038\/s41598-017-16743-1. PubMed Zhao M, et al. 2019. Cell Rep. 26:652. PubMed Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed Niss K, et al. 2020. Cell Reports. 31(11):107763. PubMed Yu VWC, et al. 2017. Cell. 168:944. PubMed Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed Wang Y, et al. 2021. Signal Transduct Target Ther. 6:409. PubMed Shen Z, et al. 2021. iScience. 24:103014. PubMed Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed Dong S, et al. 2021. Nature. 591:117. PubMed Lebert-Ghali C, et al. 2016. Blood. 127: 87-90. PubMed Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed Bost P, et al. 2020. Cell. 181(7):1475-1488. PubMed Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed Zeis P, et al. 2020. Immunity. 53:775. PubMed Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed Lefkopoulos S, et al. 2020. Immunity. 53(5):934-951.e9. PubMed Zhu J et al. 2017. Cell stem cell. 20(3):374-384 . PubMed Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed Lee A, et al. 2022. Nat Commun. 13:549. PubMed\u003cbr\u003e\nRRID: AB_2563946 (BioLegend Cat. No. 100243) AB_2563947 (BioLegend Cat. No. 100244)\u003cbr\u003e\nStructure: Ig superfamily, CD3\/TCR, 20 kD\u003cbr\u003e\nDistribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells\u003cbr\u003e\nFunction: Antigen recognition, TCR signal transduction, T cell activation\u003cbr\u003e\nLigand\/Receptor: Peptide antigen\/MHC-complex\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263.\u003cbr\u003e\nGene ID: 12502\u003cbr\u003e\nUniProt: View information about CD3 on UniProt.org\u003cbr\u003e\nClone: 17A2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T cell antigen receptor complex, T3\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860821790889,"sku":"100244","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-100244","provider":"Iright","version":"1.0","type":"link"}