{"product_id":"biolegend-100326","title":"Biolegend, 100326, PerCP anti-mouse CD3ε Antibody, 100μg","description":"\u003cp\u003eCD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α\/β or γ\/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide\/MHC antigen complex.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The CD3ε antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32, and in vivo T cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin \u0026lt;0.01 EU\/µg).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block) Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ) Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB) Payer E, et al. 1991. J. Immunol. 146:2536. (IF) Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD) Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ) Henrickson M, et al. 1995. Transplantation 60:828. (Deplete) Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete) Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete) Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block) Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ) Ko SY, et al. 2005. J. Immunol. 175:3309. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F) Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F) Wang W, et al. 2007. J. Immunol. 178:4885. (Activ) Xiao S, et al. 2007. J. Exp. Med. 204:1691. Chappaz S, et al. 2007. Blood doi:10.1182\/blood-2007-02-074245. (FC) PubMed. Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed Guo Y, et al. 2008. Blood 112:480. PubMed Kenna TJ, et al. 2008. Blood 111:2091. Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed Schulteis RD, et al. 2008. Blood 295:G273. PubMed Qi Q, et al. 2009. Blood 114:564. PubMed Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed Yan J, et al. 2014. Vaccine. 32:2833. PubMed Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)\u003cbr\u003e\nProduct Citations: Könnecke I, et al. 2014. Bone. 64c:155. PubMed Cabrera-Mora M, et al. 2016. J Immunol. 197: 2748 - 2761. PubMed Li P, et al. 2020. J Cell Mol Med. 24:10151. PubMed Cha JH et al. 2018. Molecular cell. 71(4):606-620 . PubMed Xu X, et al. 2020. Chin Med. 15:33. PubMed Li X, et al. 2023. J Clin Invest. 133:. PubMed Chen F, et al. 2023. Acta Biomater. 164:387. PubMed Bettke JA, et al. 2022. Infect Immun. 90:e0007022. PubMed Zhang H, et al. 2022. Eur J Immunol. 52:978. PubMed Ajay AK, et al. 2022. Cell Rep. 38:110473. PubMed AbuEid M, et al. 2021. iScience. 24(6):102653. PubMed Iwamoto H et al. 2018. Cell metabolism. 28(1):104-117 . PubMed Ferrere G, et al. 2021. JCI Insight. 6:. PubMed Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed Fasbender F, et al. 2017. Front Immunol. 0.88125. PubMed Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed Neumann AM et al. 2017. PloS one. 12(11):e0187330 . PubMed Xie X, et al. 2010. Proc Natl Acad Sci U S A. 107:8754. PubMed Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed Wang LT, et al. 2021. STAR Protocols. 2(1):100337. PubMed Hsu JM, et al. 2018. Nat Commun. 9:1908. PubMed Markov SD, et al. 2021. Mol Cancer Ther. 20:2457. PubMed Ma YS, et al. 2021. Mol Ther Oncolytics. 484:20. PubMed Cha H, et al. 2010. J Immunol. 184:6799. PubMed Ito C, et al. 2015. PLoS One. 10: e0140808. PubMed Pérez-Girón J, et al. 2015. J Vis Exp. 100: 52803. PubMed Chang J, et al. 2010. PLoS One. 5:e12925. PubMed Liu S, et al. 2020. Cell Host \u0026amp; Microbe. 26(6):779-794.e8.. PubMed Aguilera M, et al. 2021. Int J Mol Sci. 22:. PubMed Daneshmandi S, et al. 2021. Elife. 10:. PubMed Schreiber K, et al. 2020. Cancer Immunol Res. 8:192. PubMed Wang LT, et al. 2020. Cell Rep. 32:108188. PubMed Murdock BJ, et al. 2021. JCI Insight. 6:. PubMed Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed Li Z, et al. 2020. Front Cell Dev Biol. 8:572689. PubMed Terp MG, et al. 2021. Mol Oncol. 15:3299. PubMed Li X, et al. 2017. Front Immunol. 8:1186. PubMed Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed Nakao R, et al. 2022. NPJ Vaccines. 7:153. PubMed Stack G, et al. 2015. PLoS Pathog. 11:1004641. PubMed Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed\u003cbr\u003e\nRRID: AB_893319 (BioLegend Cat. No. 100325) AB_893317 (BioLegend Cat. No. 100326)\u003cbr\u003e\nStructure: Ig superfamily, forms CD3\/TCR complex with CD3δ, γ and ζ subunits and TCR (α\/β and γ\/δ), 20 kD\u003cbr\u003e\nDistribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells\u003cbr\u003e\nFunction: TCR signal transduction, T cell activation, antigen recognition\u003cbr\u003e\nLigand\/Receptor: Peptide antigen\/MHC-complex\u003cbr\u003e\nCell Type: NKT cells, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, TCRs\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263.\u003cbr\u003e\nGene ID: 12501\u003cbr\u003e\nUniProt: View information about CD3epsilon on UniProt.org\u003cbr\u003e\nClone: 145-2C11\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD3ε, T3, CD3\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860804260009,"sku":"100326","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-100326","provider":"Iright","version":"1.0","type":"link"}