{"product_id":"biolegend-100624","title":"Biolegend, 100624, PerCP\/Cyanine5.5 anti-mouse CD5 Antibody, 100μg","description":"\u003cp\u003eCD5 is a 67 kD protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature α\/β T cells express high levels of CD5, very few γ\/δ T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse thymus or spleen\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2 of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP) Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC) Bourdeau A, et al. 2007. Blood doi:10.1182\/blood-2006-08-044370.\u003cbr\u003e\nProduct Citations: Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed Simpson AP, et al. 2022. Cell Rep. 40:111099. PubMed Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed Català C, et al. 2022. iScience. 25:105078. PubMed Kobayashi T, et al. 2019. Cell. 176:982. PubMed Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed Bennion BG, et al. 2020. Cell Reports. 31(11):107771. PubMed Snyder LM, et al. 2022. Immunohorizons. 6:660. PubMed Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed Nasarre P, et al. 2021. Cancers (Basel). 13: . PubMed Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed Peng V, et al. 2022. Nat Immunol. 23:619. PubMed Teng F, et al. 2021. Cell Rep. 37:110051. PubMed Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed Borrelli MR, et al. 2020. Stem Cells Transl Med. 1.347916667. PubMed Goc J, et al. 2021. Cell. 184:5015. PubMed\u003cbr\u003e\nRRID: AB_2563432 (BioLegend Cat. No. 100623) AB_2563433 (BioLegend Cat. No. 100624)\u003cbr\u003e\nStructure: Member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD\u003cbr\u003e\nDistribution: Thymocytes, T cells, B-1 cells\u003cbr\u003e\nFunction: Negative regulator of T-B cell interaction\u003cbr\u003e\nLigand\/Receptor: CD72, gp35-37\u003cbr\u003e\nCell Type: B cells, T cells, Thymocytes\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kipps TJ. 1988. Adv. Immunol. 47:117. 3. Antin JH, et al. 1985. J. Immunol. 136:505. 4. Tarakhovsky A, et al. 1995. Science 269:535.\u003cbr\u003e\nGene ID: 12507\u003cbr\u003e\nUniProt: View information about CD5 on UniProt.org\u003cbr\u003e\nClone: 53-7.3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Lyt-1, Ly-1, T1, Tp67, Ly-12\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862441611433,"sku":"100624","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-100624","provider":"Iright","version":"1.0","type":"link"}