{"product_id":"biolegend-101331","title":"Biolegend, 101331, Brilliant Violet 421™ anti-mouse CD16\/32 Antibody, 125microl","description":"\u003cp\u003eCD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16\/CD32 are expressed on B cells, monocytes\/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.\u003cbr\u003e\n125microl\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Sorted pre-B cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: µg sizes: 0.2 mg\/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Clone 93 can be used for blocking of CD16\/CD32 interactions with the Fc domain of immunoglobulins, but is not the same clone as 2.4G2.The 93 mAb is specific to the common epitope of CD16\/CD32. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and blocking of Fc-mediated reactions in functional studies2,4,23. It is useful for blocking non-specific binding of immunoglobulin to Fc receptors. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with purified anti-CD16\/CD32 antibody (=1.0 µg per 106 cells in 100 µL volume) for 5-10 minutes on ice prior to immunostaining. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101330) (Endotoxin \u0026lt;0.01 EU\/µg, Azide-Free, 0.2 µm filtered).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Personal communication (IP) Oliver AM, et al. 1999. Hybridoma 18:113. (Block) Brummel R and Lenert P. 2005. J. Immunol. 174:2429. Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (Block) Clements JL, et al. 2006. J. Immunol. 177:905. Mohamed W, et al. 2010. Infect Immun. 78:3306. PubMed Ouchi T, et al. 2011. J. Exp Med. 208:2607. PubMed Kmieciak M, et al. 2011. J. Vis. Exp. 47:2381. PubMed Yamazaki S, et al. 2012. PLoS One. 7:e51665. PubMed Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed Azuma M, et al. 2012. Oncoimmunology. 1:581. PubMed Koon HW, et al. 2013. J. Vis. Exp. 68:4208. PubMed Hegde VL, et al. 2013. J Biol Chem. 288:36810. PubMed Huang J, et al. 2013. J. Immunol Methods. 387:254. PubMed Dutow P, et al. 2014. J Infect Dis. PubMed Fan Y, et al. 2014. J Exp Med. 211:313. PubMed Huang HN, et al. 2014. Antimicrob Agents Chemother. 58:1538. PubMed Takei S, et al. 2014. Vaccine. 32:3066. PubMed Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed Cekanaviciute E, et al. 2014. J Immunol. 193:139. PubMed Kimura T, et al. 2014. Int Immunol. 26:697. PubMed Everad A, et al. 2014. Nat Commun. 5:5648. PubMed Cenci E, et al. 2006. J. Leuko. Biol. 79(1):40-5. (Block)\u003cbr\u003e\nProduct Citations: Zhang H, et al. 2023. Pharmaceutics. 15:. PubMed Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed Blankenhaus B, et al. 2019. Mol Metab. 24:64. PubMed Kim SP, et al. 2021. Cell Reports. 36(9):109626. PubMed Tyrkalska SD, et al. 2019. Immunity. 51:50. PubMed Yang J, et al. 2022. Commun Biol. 5:124. PubMed Wu L, et al. 2022. Theranostics. 12:842. PubMed Kalafati L, et al. 2020. Cell. 183:771. PubMed Utz SG, et al. 2020. Cell. 181(3):557-573. PubMed Yoshinaga M, et al. 2022. Nat Commun. 13:6435. PubMed\u003cbr\u003e\nRRID: AB_2562188 (BioLegend Cat. No. 101331) AB_2650889 (BioLegend Cat. No. 101332)\u003cbr\u003e\nStructure: Ig superfamily, 40-60 kD\u003cbr\u003e\nDistribution: B cells, monocyte\/macrophages, NK cells, neutrophils, mast cells, dendritic cells\u003cbr\u003e\nFunction: Low affinity receptors for IgG\u003cbr\u003e\nLigand\/Receptor: IgG\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, Fc Receptors\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Unkeless JC. 1989. J. Clin. Invest. 83:355. 3. Qiu WQ, et al. 1990. Science 248:732.\u003cbr\u003e\nGene ID: 1413014131\u003cbr\u003e\nUniProt: View information about CD16\/32 on UniProt.org\u003cbr\u003e\nClone: 93\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Fcγ R III\/II, Ly-17\u003cbr\u003e\nIsotype: Rat IgG2a, λ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862502002857,"sku":"101331","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-101331","provider":"Iright","version":"1.0","type":"link"}