{"product_id":"biolegend-102420","title":"Biolegend, 102420, PerCP\/Cyanine5.5 anti-mouse CD31 Antibody, 100μg","description":"\u003cp\u003eCD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes\/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (α v β 3 integrin, CD51\/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: C3H\/HeJ mouse hematopoietic progenitor cell line 3\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin \u0026lt; 0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102412).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Baldwin HS, et al. 1994. Development 120:2539. (IP, Block) DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block) Rosenblum WI, et al. 1996. Stroke 27:709. (Block) Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block) Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC) Wiewrodt R, et al. 2002. Blood 99:912. (IHC) McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed Chacko AM, et al. 2012. PLoS One 7:e34958. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed\u003cbr\u003e\nProduct Citations: Zhang Q, et al. 2022. Nat Metab. 4:1055. PubMed Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed Wang L, et al. 2022. Cell Metab. 34:75. PubMed Boyd DF, et al. 2020. Nature. 587:466. PubMed Sun C, et al. 2020. Cell Stem Cell. 26(5):707-721. PubMed Lin J, et al. 2021. Front Immunol. 12:796260. PubMed Shen H, et al. 2022. Nat Commun. 13:6030. PubMed Evans R, et al. 2019. Cell Rep. 27:1967. PubMed Angelidis I, et al. 2019. Nat Commun. 10:963. PubMed Emgård J, et al. 2018. Immunity. 143:419. PubMed Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed Prados A, et al. 2021. Nat Immunol. 22:510. PubMed Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed Li X, et al. 2022. Bone Res. 10:46. PubMed Parker KR, et al. 2020. Cell. 183(1):126-142.e17. PubMed Hepler C et al. 2018. eLife. 7 pii: e39636. PubMed Shao M, et al. 2021. Cell Stem Cell. 28(4):685-701.e7. PubMed Joffin N, et al. 2021. Cell Stem Cell. 28(4):702-717.e8. PubMed Walsh SM, et al. 2021. eLife. 10:00. PubMed van Gastel N, et al. 2020. Cell Metabolism. 32(3):391-403.e6. PubMed Shan B, et al. 2020. Nat Metab. 1332:2. PubMed Francipane MG, et al. 2020. Am J Pathol. 190:252. PubMed Wang S, et al. 2021. Commun Biol. 22:. PubMed Kato K, et al. 2018. Nat Commun. 48:120. PubMed Mesnieres M, et al. 2021. Cell Reports. 36(8):109618. PubMed Okada R, et al. 2021. EBioMedicine. 67:103345. PubMed Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed Tal R, et al. 2019. Biol Reprod. 100:1228. PubMed Rai S, et al. 2022. Nat Commun. 13:5346. PubMed Wang Y, et al. 2020. Theranostics. 6.736111111. PubMed Prados A, Kollias G, Koliaraki V 2016. Sci Rep. 6: 33027. PubMed Eilken H, et al. 2017. Nat Commun. . 10.1038\/s41467-017-01738-3. PubMed Noll J, et al. 2014. Haematologica. 99:163. PubMed Li CY, et al. 2022. Int J Mol Sci. 23:. PubMed\u003cbr\u003e\nRRID: AB_10612742 (BioLegend Cat. No. 102419) AB_10613644 (BioLegend Cat. No. 102420)\u003cbr\u003e\nStructure: Ig superfamily, 130-140 kD\u003cbr\u003e\nDistribution: Endothelial cells, platelets, granulocytes, monocytes\/macrophages, dendritic cells, T and B cell subsets\u003cbr\u003e\nFunction: Adhesion\u003cbr\u003e\nLigand\/Receptor: CD38, αV\/β3 integrin\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. DeLisser HM, et al. 1994. Immunol. Today 15:490. 3. Newman PJ, et al. 1990. Science 247:1219.\u003cbr\u003e\nGene ID: 18613\u003cbr\u003e\nUniProt: View information about CD31 on UniProt.org\u003cbr\u003e\nClone: 390\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PECAM-1, EndoCAM\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862383808681,"sku":"102420","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-102420","provider":"Iright","version":"1.0","type":"link"}