{"product_id":"biolegend-104734","title":"Biolegend, 104734, PE\/Cyanine7 anti-mouse CD80 Antibody, 100μg","description":"\u003cp\u003eCD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes\/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nReported Reactivity: Dog\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: CHO cell line transfected with mouse B7 (CD80)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104747-104752).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC) Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP) Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block) Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC) Ma XT, et al. 2006. Cancer Res. 66:1169. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) Lawson BR, et al. 2007. J. Immunol. 178:5366. Turnquist HR, et al. 2007. J. Immunol. 178:7018. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed\u003cbr\u003e\nProduct Citations: Chen X, et al. 2023. Adv Mater. 35:e2210440. PubMed Li Y, et al. 2023. Adv Mater. 35:e2208923. PubMed Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed Kaur N, et al. 2023. Heliyon. 9:e14952. PubMed Deng G, et al. 2023. Cancers (Basel). 15:. PubMed Wang W, et al. 2022. Dev Cell. 57:228. PubMed Yue Y, et al. 2022. Nat Biomed Eng. 6:898. PubMed Manda-Handzlik A, et al. 2022. Int J Mol Sci. 23: . PubMed Wang W, et al. 2022. Cell Rep. 41:111582. PubMed Kumpunya S, et al. 2023. Front Immunol. 13:1010764. PubMed Liu H, et al. 2023. Cell Death Differ. :. PubMed Zenke S, et al. 2022. Nat Commun. 13:6459. PubMed McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed Cullis J, et al. 2017. Cancer Immunol Res. 5:182. PubMed Padonou F, et al. 2022. EMBO Rep. 23:e53576. PubMed Chen J, et al. 2022. J Nanobiotechnology. 20:283. PubMed Paraiso HC, et al. 2018. J Neuroinflammation. 0.694444444. PubMed Teng ZX, et al. 2022. J Inflamm Res. 15:3187. PubMed Wang S, et al. 2018. Biomaterials. 187:28:00. PubMed Cheng K, et al. 2021. Nat Commun. 12:2041. PubMed Utz B, et al. 2020. Sci Rep. 10:18733. PubMed Akazawa S, et al. 2021. Diabetologia. 64:878. PubMed Haikala HM, et al. 2019. Nat Commun. 10:620. PubMed Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed Ding X, et al. 2019. J Am Heart Assoc. 8:e011211. PubMed Ishikura N, et al. 2022. Oncol Rep. 47:. PubMed Wang X, et al. 2020. Nat Commun. 4.505555556. PubMed Guo S, et al. 2021. Sci Rep. 11:23745. PubMed Kiyohara H, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:135. PubMed Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed Zhang W, et al. 2021. Front Immunol. 12:768435. PubMed Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed Siolas D, et al. 2021. Cell Reports. 36:109578. PubMed Li H, et al. 2022. iScience. 25:104481. PubMed Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed\u003cbr\u003e\nRRID: AB_2563112 (BioLegend Cat. No. 104733) AB_2563113 (BioLegend Cat. No. 104734)\u003cbr\u003e\nStructure: Ig superfamily, 60 kD\u003cbr\u003e\nDistribution: Macrophages, activated B cells and T cells, dendritic cells\u003cbr\u003e\nFunction: T cell costimulation\u003cbr\u003e\nLigand\/Receptor: CD28 (stimulatory), CD152(CTLA4) (inhibitory)\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Macrophages, T cells, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Linsley PS, et al. 1991. J. Exp. Med. 174:561. 3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.\u003cbr\u003e\nGene ID: 12519\u003cbr\u003e\nUniProt: View information about CD80 on UniProt.org\u003cbr\u003e\nClone: 16-10A1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: B7-1, B7, Ly-53\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860815335593,"sku":"104734","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-104734","provider":"Iright","version":"1.0","type":"link"}