{"product_id":"biolegend-105404","title":"Biolegend, 105404, PE anti-mouse\/human Helios Recombinant Antibody, 100tests","description":"\u003cp\u003eHelios is a member of the Ikaros family of zinc finger transcription factors. It contains a C-terminal region composed of 2 zinc-finger domains that mediate dimerization between the family members. Helios was originally cloned from a mouse thymoma line. It is mainly expressed in peripheral T cells and thymocytes. It is found at high levels in a subpopulation of regulatory T cells. Helios plays an important role in T cell development and homeostasis. Overexpression of Helios profoundly alters αβ T cell differentiation and activation. It has been determined that silencing of Helios in B cells is critical for maintaining normal B cell function. Helios is also involved in tumor immunity.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Mouse, Human\u003cbr\u003e\nAntibody Type: Recombinant\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Clone QA20A52 is able to completely block the staining of clone 22F6 on target cells\u003cbr\u003e\nRRID: AB_2894413 (BioLegend Cat. No. 105403) AB_2894413 (BioLegend Cat. No. 105404)\u003cbr\u003e\nStructure: A zinc finger nuclear transcription factor of the Ikaros family, containing 2 zinc-finger domains in C-terminal region.\u003cbr\u003e\nDistribution: Regulatory T cells (Tregs), conventional T cells, thymocytes.\u003cbr\u003e\nFunction: Plays an important role in T cell development and homeostasis, involved in tumor immunity.\u003cbr\u003e\nCell Type: T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Adaptive Immunity, Cell Biology, Immunology, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Kelly CM, et al. 1998. Curr Biol. 8:508. 2. Dovat S, et al. 2005. J Immunol. 175:3508. 3. Cortes M, et al. 1999. Curr Opin Immunol. 11:167. 4. Cai Q, et al. 2009. J Immunol. 183:2303. 5. Zhang Z, et al. 2007. Blood. 109:2190. 6. Hahm K, et al. 1998. Genes Dev. 12:782.\u003cbr\u003e\nGene ID: 2277922807\u003cbr\u003e\nUniProt: View information about Helios on UniProt.org\u003cbr\u003e\nClone: QA20A52\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IKZF2, IKAROS family zinc finger 2, ANF1A2, ZNF1A2, ZNFN1A2\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866192040105,"sku":"105404","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-105404","provider":"Iright","version":"1.0","type":"link"}