{"product_id":"biolegend-105712","title":"Biolegend, 105712, Alexa Fluor® 647 anti-mouse CD106 Antibody, 100μg","description":"\u003cp\u003eCD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 (α 4 \/β 1 integrin) and LPAM-1 (α 4 \/β 7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse preadipose cell line PA6\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested IHC-F, 3D IHC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume. For immunohistochemical staining on frozen tissue sections, the suggested use is 2.5 - 10 µg\/mL. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for other applications. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm \/ 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining2,3,5-7 of acetone-fixed frozen sections, blocking4,5,8 of ligand binding in vitro and in vivo, immunoprecipitation1 , and spacial biology (IBEX)11,12. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105727 \u0026amp; 105728).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. (IP) Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC) Ishiyama N, et al. 1998. Pathobiology 66:274. (IHC) Kinashi T, et al. 1994. Blood Cells 20:25. (Block) Baron JL, et al. 1994. J. Clin. Invest. 93:1700. (Block IHC) Buck CA, et al. 1996. Cell Adhes. Commun. 4:69. (IHC) Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC) Meunier MC, et al. 2005. Nature Medicine 11:1222. (Block) PubMed Monnier J, et al. 2012. J. Immunol. 189:956. PubMed Motohashi N, et al. 2013. J Cell Sci. 126:2678. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed Krausgruber T, et al. 2020. Nature. 583:296. PubMed Philpott J, et al. 2022. Immunohorizons. 6:366. PubMed Adamiak M, et al. 2015. Leukemia. . PubMed Sung SJ, et al. 2020. Bio Protoc. 10:e3663. PubMed Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed Wang C, et al. 2021. Cell Rep. 37:110021. PubMed Nakagawa R, et al. 2013. J Immunol. 190:3309. PubMed Onder L et al. 2017. Immunity. 47(1):80-92 . PubMed Bonnardel J, et al. 2019. Immunity. 51:638. PubMed Pietronigro E, et al. 2019. Sci Rep. 9:12055. PubMed Bolkhovitina EL, et al. 2022. Int J Mol Sci. 23: . PubMed Nanou A, et al. 2021. Cell Reports. 35(8):109168. PubMed Armaka M, et al. 2018. Nat Commun. 9:618. PubMed Sun D, et al. 2020. PLoS Pathog. 16:e1008361. PubMed\u003cbr\u003e\nRRID: AB_493430 (BioLegend Cat. No. 105711) AB_493429 (BioLegend Cat. No. 105712)\u003cbr\u003e\nStructure: Ig superfamily, 47 kD\u003cbr\u003e\nDistribution: Bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells\u003cbr\u003e\nFunction: Adhesion\u003cbr\u003e\nLigand\/Receptor: VLA-4 (α4\/β1 integrin) and LPAM-1 (α4\/β7 integrin)\u003cbr\u003e\nCell Type: Dendritic cells, Endothelial cells, Mesenchymal Stem Cells\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. 3. Bevilacquea MP. 1993. Annu. Rev. Immunol. 11:767. 4. Koni PA, et al. 2001. J. Exp. Med. 193:741.\u003cbr\u003e\nGene ID: 22329\u003cbr\u003e\nUniProt: View information about CD106 on UniProt.org\u003cbr\u003e\nClone: 429 (MVCAM.A)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: VCAM-1, INCAM-110\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862402584745,"sku":"105712","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-105712","provider":"Iright","version":"1.0","type":"link"}