{"product_id":"biolegend-105803","title":"Biolegend, 105803, Biotin anti-mouse CD117 (c-Kit) Antibody, 50μg","description":"\u003cp\u003eCD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and\/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse bone marrow mast cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC) Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC) Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC) Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC) Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Drobek A, et al. 2015. J Immunol. 195: 3416 - 3426. PubMed Kuroki S, et al. 2020. Stem Cell Reports. 0.919444444. PubMed Peng YJ, et al. 2023. Development. 150:. PubMed Gargaro M, et al. 2022. Immunity. 55:1032. PubMed Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed Ye B, et al. 2022. Cell Mol Immunol. 19:619. PubMed Larsen SB, et al. 2021. Cell Stem Cell. 28:1758. PubMed Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed Onodera T, et al. 2019. J Immunol. 203:3282. PubMed Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed Adam RC et al. 2018. Cell stem cell. 22(3):398-413 . PubMed Rai S, et al. 2014. PLoS One. 9:109441. PubMed Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed Onodera T, et al. 2021. Immunity. 54:2385. PubMed Kandalla P, et al. 2016. J Exp Med. 213: 2269 - 2279. PubMed Fujii T, et al. 2021. Bone Res. 9:4. PubMed Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed Laurin M, et al. 2019. Elife. 8. PubMed Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed Bae S, et al. 2021. Cell Reports. 35(11):109264. PubMed\u003cbr\u003e\nRRID: AB_313212 (BioLegend Cat. No. 105803) AB_313213 (BioLegend Cat. No. 105804)\u003cbr\u003e\nStructure: Ig superfamily, 145 kD\u003cbr\u003e\nDistribution: Hematopoietic stem cells, AML, mast cells\u003cbr\u003e\nFunction: Growth factor receptor, tyrosine kinase\u003cbr\u003e\nLigand\/Receptor: Stem Cell Factor (SCF)\u003cbr\u003e\nCell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells\u003cbr\u003e\nBiology Area: Immunology, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Galli SJ, et al. 1994. Adv. Immunol. 55:1. 3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759. 4. Besmer P, et al. 1986. Nature 320:415. 5. Witte ON. 1990. Cell 63:5.\u003cbr\u003e\nGene ID: 16590\u003cbr\u003e\nUniProt: View information about CD117 on UniProt.org\u003cbr\u003e\nClone: 2B8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: c-KIT, Stem Cell Factor Receptor (SCFR)\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866540495017,"sku":"105803","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-105803","provider":"Iright","version":"1.0","type":"link"}