{"product_id":"biolegend-106314","title":"Biolegend, 106314, PE\/Cyanine7 anti-mouse CD152 Antibody, 100μg","description":"\u003cp\u003eCD152, also known as CTLA-4 or Ly-56, is a 33 kD member of the immunoglobulin superfamily. It is expressed on activated T and B lymphocytes. CD152 is similar to CD28 in amino acid sequence, structure, and genomic organization and these two receptors share common B7 family counter-receptors (B7-1, B7-2). Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses. CD152 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: Mouse CTLA-4-mouse IgG2a fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: The UC10-4B9 antibody can enhance T cell co-stimulation by blocking CTLA-4 interactions with the B7 co-receptors, favoring CD28 interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro stimulation, in vitro and in vivo blocking1-4 of ligand binding, and as ELISA capture antibody5. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16\/CD32, clone 93 (Cat. No. 101301\/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The Ultra LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 106327).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Walunas TL, et al. 1994. Immunity 1:405. (Block, IP) Cilio CM, et al. 1998. J. Exp. Med. 188:1239. (Block) Issazadeh S, et al. 1999. J. Immunol. 162:761. (Block) McCoy K, et al. 1997. J. Exp. Med. 186:183. (Block) Hsu HC, et al. 2007. J. Immunol. 178:5357. (ELISA Capture) Sugita S, et al. 2010. Invest. Ophthalmol. Vis. Sci. 51:5783. PubMed\u003cbr\u003e\nProduct Citations: Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed Sanchez-Moral L, et al. 2023. EBioMedicine. 91:104555. PubMed Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed Potluri HK, et al. 2022. J Immunother Cancer. 10:. PubMed Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed Naing A et al. 2019. Cell reports. 26(5):1242-1257 . PubMed Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed M H, et al. 2016. Sci Rep. 6: 20588. PubMed Kim D, et al. 2020. Immunity. 53(3):581-596.e5. PubMed Bommareddy PK, et al. 2019. J Biol Methods. 6:2. PubMed Truckenbrod EN, et al. 2021. Elife. 10:. PubMed\u003cbr\u003e\nRRID: AB_2564237 (BioLegend Cat. No. 106313) AB_2564238 (BioLegend Cat. No. 106314)\u003cbr\u003e\nStructure: Ig superfamily, 33 kD\u003cbr\u003e\nDistribution: Activated T cells and B cells\u003cbr\u003e\nFunction: Negative regulator of T cell activation\u003cbr\u003e\nLigand\/Receptor: CD80 (B7-1), CD86 (B7-2)\u003cbr\u003e\nCell Type: B cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Allison JP, et al. 1995. Science 270:932. 3. Waterhouse P, et al. 1995. Science 270:985. 4. Linsley PS, et al. 1991. J. Exp. Med. 174:561.\u003cbr\u003e\nGene ID: 12477\u003cbr\u003e\nUniProt: View information about CD152 on UniProt.org\u003cbr\u003e\nClone: UC10-4B9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Cytotoxic T Lymphocyte-Associated Antigen-4 (CTLA-4), Ly-56\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864068018345,"sku":"106314","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-106314","provider":"Iright","version":"1.0","type":"link"}