{"product_id":"biolegend-108903","title":"Biolegend, 108903, Biotin anti-mouse CD49b (pan-NK cells) Antibody, 50μg","description":"\u003cp\u003eDX5 antigen has been recently characterized as CD49b. It is a 150 kD integrin α chain also known as α 2 integrin, VLA-2 α chain, and integrin α 2 chain. CD49b non-covalently associates with CD29 (β 1 integrin) to form the CD49b\/CD29 complex known as VLA-2, a receptor for collagen and laminin. CD49b is expressed on platelets, the majority of NK cells, NKT cells, and a small subset of CD8+ T cells (this population can be significantly increased following viral infection). DX5 is used for the identification and isolation of NK cells, and is especially useful for identifying NK cells in mice lacking the NK1.1 antigen.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: IL-2-propagated NK1.1+ cells from C57BL\/6 mice\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The DX5 clone detects cells expressing relatively high levels of CD49b and may not be useful for the detection of cells expressing low levels of CD49b. DX5 does not block NK cell killing or binding to collagen in vitro. Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity2 and immunohistochemical staining5 of formalin-fixed and paraffin-embedded tissue sections as well as immunohistochemical staining of acetone-fixed frozen sections10. The binding of DX5 antibody to splenic NK cells can be blocked by HMa2 antibody.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Arase H, et al. 2001. J. Immunol. 167:1141. (FC) Sepulveda H, et al. 1999. J. Immunol. 163:1133. Norian LA and Allen PM. 2004. J. Immunol. 173:835. (FC) Andoniou CE, et al. 2005. Nature Immunology 6:1011. Oertelt S, et al. 2006. J. Immunol. 177:1655. (IHC) PubMed Bourdeau A, et al. 2007. Blood doi:10.1182\/blood-2006-08-044370. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed Busche A, et al. 2011. J. Immunol. 186:2918. PubMed Kim HR, et al. 2011. Nephrology 16:545. (IHC) PubMed Seyoum B, et al. 2011. Vaccine. 29:8002. PubMed Younos IH, et al. 2012. Int Immunopharmacol. 13:245. PubMed Honjo K, et al. 2012. PNAS. PubMed. Huang HN, et al. 2013. Biomaterials. 34:10151. PubMed\u003cbr\u003e\nProduct Citations: Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed McGee MC, et al. 2022. Bio Protoc. 12:. PubMed Miyake K, et al. 2023. Nat Commun. 14:2694. PubMed Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed Chanda MK, et al. 2022. J Immunol Methods. 506:113290. PubMed Tomiaki C, et al. 2022. Front Immunol. 13:1014462. PubMed Boyd DF, et al. 2020. Nature. 587:466. PubMed Rosenheinrich M, et al. 2015. PLoS One. 10: 0136290. PubMed Elsner R, et al. 2015. PLoS Pathog. 10: 1004976. PubMed Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed Guo F, et al. 2022. Front Immunol. 13:833100. PubMed de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed Browning LM, et al. 2020. Cell Rep. 33:108219. PubMed Li W, et al. 2012. J Virol. 86:12304. PubMed Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed Cravens P, et al. 2016. PLoS One. 11:e0165248. PubMed Van de Velde L, et al. 2016. Cell Rep. 17:2247-2258. PubMed Liu X, et al. 2021. eLife. 0.416666666666667. PubMed Suah AN, et al. 2021. J Clin Invest. 131:. PubMed Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed Matsumura T et al. 2019. Cell Rep. 27(2):561-571 . PubMed Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed Chauveau A, et al. 2020. Immunity. 52:794. PubMed Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed Au-Yeung BB, et al. 2017. J Immunol. 198:2445. PubMed Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed Akkaya B, et al. 2017. J Immunol Methods. 441:67. PubMed Yang E, et al. 2016. J Immunol. 197: 934 - 941. PubMed Iki M, et al. 2016. Blood. 128(25):2909-2918. PubMed Tsutsui H, et al. 2017. J Biol Chem. 292(3):1061-1067. PubMed Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed Lutz J, et al. 2015. Nat Commun. 6: 8575. PubMed Pisano F, et al. 2014. PLoS One. 9:103541. PubMed Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed Kashiwakura Y, et al. 2020. Clinical \u0026amp; Experimental Immunology. 202(1):119-135. PubMed Jorge Domínguez‐Andrés et al. 2017. Immunity. 46(6):1059-1072 . PubMed Celias DP, et al. 2022. STAR Protoc. 3:101464. PubMed\u003cbr\u003e\nRRID: AB_313410 (BioLegend Cat. No. 108903) AB_313411 (BioLegend Cat. No. 108904)\u003cbr\u003e\nStructure: Integrin α chain, 150 kD\u003cbr\u003e\nDistribution: NK cells, subset of T cells\u003cbr\u003e\nFunction: Adhesion\u003cbr\u003e\nLigand\/Receptor: Collagen, laminin\u003cbr\u003e\nCell Type: NK cells, T cells\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Arase H, et al. 2001. J. Immunol. 167:1141. 2. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 3. Sasaki K, et al. 2003. Int. Immunol. 15:701. 4. Inoue O, et al. 2003. J. Cell Biol. 160:769.\u003cbr\u003e\nGene ID: 16398\u003cbr\u003e\nUniProt: View information about CD49b on UniProt.org\u003cbr\u003e\nClone: DX5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: α2 integrin, VLA-2 α chain, DX5, Integrin α2 chain, ITGA2\u003cbr\u003e\nIsotype: Rat IgM, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863061975209,"sku":"108903","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-108903","provider":"Iright","version":"1.0","type":"link"}