{"product_id":"biolegend-109824","title":"Biolegend, 109824, APC\/Cyanine7 anti-mouse CD45.2 Antibody, 100μg","description":"\u003cp\u003eCD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB\/c, CBA\/Ca, CBA\/J, C3H\/He, C57BL, C57BR, C57L, C58, DBA\/1, DBA\/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: B10.S mouse thymocytes and splenocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) Suzuki K, et al. 2000. Immunity 13:691. (IHC) Shen FW, et al. 1986. Immunogenetics 24:146. (IP) Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837. Pascal V, et al. 2007. J. Immunol. 179:1751. Burman AC, et al. 2007. Blood 110:1064. Kincaid EZ, et al. 2007. J. Immunol. 179:3187. Phan TG, et al. 2007. Nature Immunol. 8:992. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed Wen T, et al. 2013. PNAS. 110:6067. PubMed Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed\u003cbr\u003e\nProduct Citations: Chang D, et al. 2020. Immunity. 53(3):614-626.e4. PubMed Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed Gravano D, et al. 2010. PLoS One. 5:e13528. PubMed Xu H, et al. 2023. Elife. 12:. PubMed Chang D, et al. 2023. Front Immunol. 14:1105145. PubMed Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed Yamazaki S, et al. 2022. Mucosal Immunol. :. PubMed Leung W, et al. 2022. Cancer Discov. 12:1782. PubMed Shen Z, et al. 2022. EBioMedicine. 85:104285. PubMed Yoo HJ, et al. 2022. Cell Rep. 41:111598. PubMed Pleuger C, et al. 2022. Elife. 11: . PubMed Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed Kasuya T, et al. 2023. Sci Rep. 13:1653. PubMed Cheng H, et al. 2023. Nat Metab. 5:314. PubMed Ernst MPT, et al. 2023. Hemasphere. 7:e824. PubMed Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed Sohn J, et al. 2022. Blood Adv. 6:2557. PubMed Huang J, et al. 2021. Immunity. 54(4):673-686.e4. PubMed Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed Poh AR, et al. 2017. Cancer Cell. 31:563. PubMed Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed Zhou J, et al. 2019. Immunity. 50:403. PubMed Adrover JM, et al. 2022. JCI Insight. 7:. PubMed Iturri L, et al. 2021. Immunity. . PubMed McLane LM, et al. 2021. Cell Reports. 35(6):109120. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Tran I, et al. 2013. J Clin Invest. 123:1590. PubMed Kang S, et al. 2011. J Exp Med. 208:747. PubMed Bowers E, et al. 2018. Nat Med. 24:95. PubMed Drajac C, et al. 2021. Mucosal Immunol. 14:949. PubMed Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed Si Y, et al. 2020. Sci Adv. 6:eaba0995. PubMed Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed Mann M, et al. 2018. Cell Rep. 25:2992. PubMed LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed Marangoni F, et al. 2021. Cell. . PubMed Xu M et al. 2018. Immunity. 48(4):787-798 . PubMed Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed Beauchamp EM, et al. 2021. Blood Cancer Discov. 6:. PubMed Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed Jeong SH, et al. 2021. Nat Commun. 12:4405. PubMed Li J, et al. 2020. Elife. 9:00. PubMed Yang Z, et al. 2020. Sci Rep. 17757:10. PubMed Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed Mintz MA, et al. 2019. Immunity. 51:310. PubMed Codina A, et al. 2019. Cell Syst. 8:136. PubMed Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed Wang W, et al. 2020. Immunity. 51(6):1102-1118.e7.. PubMed Schepanski S, et al. 2022. Nat Commun. 13:4571. PubMed Mansell E, et al. 2020. Cell Stem Cell. . PubMed Kenswil KJG, et al. 2021. Cell Stem Cell. 28(4):653-670.e11. PubMed Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 - E5714. PubMed Li H, et al. 2011. Blood. 117:697. PubMed Béguelin W, et al. 2020. Cancer Cell. 37(5):655-673. PubMed White C, et al. 2015. J Immunol. 194:697. PubMed LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed Ma W, et al. 2017. Cell Death \u0026amp; Disease. 10.1038\/cddis.2017.47. PubMed Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed Ishikura S, et al. 2021. J Cell Biochem. . PubMed Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed Bitschar K, et al. 2019. Nat Commun. 10:2730. PubMed Chen F, et al. 2022. Cell Rep. 38:110215. PubMed Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed Li C, et al. 2018. Cell. 174:285. PubMed Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed Xu C, et al. 2020. Immunity. 53(2):417-428.e4. PubMed Laubreton D, et al. 2020. Viruses. 12:00. PubMed Niven J, et al. 2019. Cell Rep. 28:21. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed Wang X, et al. 2021. Cell. 184:5357. PubMed Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed Ortega-Molina A, et al. 2021. Cell Reports. 36(2):109372. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Glodde N et al. 2017. Immunity. 47(4):789-802 . PubMed Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed Ajith A, et al. 2019. FASEB J. 33:5220. PubMed Fielding C, et al. 2022. Nat Commun. 13:543. PubMed Bretz N, et al. 2014. Immunol Lett. 161:140. PubMed Peloquin GL, et al. 2019. Respir Res. 20:123. PubMed Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed Doo DW, et al. 2020. Ther Adv Med Oncol. 12:1758835920913798. PubMed Barcia Durán JG, et al. 2021. Commun Biol. 4:406. PubMed Zheng Y, et al. 2022. Transl Res. :. PubMed Effern M, et al. 2020. Immunity. 53(3):564-580.e9. PubMed Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed Song W, et al. 2022. Immunity. 55:290. PubMed Zhang J, et al. 2021. Stem Cell Res Ther. 12:579. PubMed\u003cbr\u003e\nRRID: AB_830788 (BioLegend Cat. No. 109823) AB_830789 (BioLegend Cat. No. 109824)\u003cbr\u003e\nStructure: Protein tyrosine phosphatase (PTP) family, 180-240 kD\u003cbr\u003e\nDistribution: All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice\u003cbr\u003e\nFunction: Phosphatase, T and B cell activation\u003cbr\u003e\nLigand\/Receptor: Galectin-1, CD2, CD3, CD4\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Suzuki K, et al. 2000. Immunity 13:691.\u003cbr\u003e\nGene ID: 19264\u003cbr\u003e\nUniProt: View information about CD45.2 on UniProt.org\u003cbr\u003e\nClone: 104\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Ly-5.2, LCA\u003cbr\u003e\nIsotype: Mouse (SJL) IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860771197097,"sku":"109824","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-109824","provider":"Iright","version":"1.0","type":"link"}