{"product_id":"biolegend-110307","title":"Biolegend, 110307, PE anti-mouse H-2D, 50μg","description":"\u003cp\u003eThe 15-5-5 antibody reacts with the H-2D k MHC class I alloantigen expressed on nucleated cells from mice of the H-2D k haplotype. H-2D k is involved in antigen presentation to T cells expressing CD3\/TCR and CD8 proteins. The 15-5-5 antibody weakly cross-reacts with H-2K d and with cells from mice of the H-2 f haplotype. Reactivity with other haplotypes ( e.g., a,b,p,q,r,s) has not been reported.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: C3H mouse splenocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1. This clone does not cross react with other haplotypes (e.g., a, b, p, q, r, s).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ozato K, et al. 1980. J. Immunol. 124:533. Yamamoto M, et al. 1998. J. Immunol. 160:2188. Houlden BA, et al. 1991. J. Immunol. 146:425. (IP) PubMed\u003cbr\u003e\nProduct Citations: Wautier MP, et al. 2007. Blood. 894:110. PubMed Litzler LC, et al. 2023. J Exp Med. :220. PubMed Lim S, et al. 2023. Investig Clin Urol. 64:74. PubMed Xie X, et al. 2007. J Virol. 81:229. PubMed Sido J, et al. 2015. J Leukoc Biol. 98: 435-447. PubMed Fang R, et al. 2007. Infect Immun . 75:3112. PubMed\u003cbr\u003e\nRRID: AB_313480 (BioLegend Cat. No. 110307)\u003cbr\u003e\nStructure: MHC class I\u003cbr\u003e\nDistribution: Nucleated cells of the H-2Dk haplotype\u003cbr\u003e\nFunction: Antigen presentation\u003cbr\u003e\nLigand\/Receptor: CD3\/TCR, CD8\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. 3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.\u003cbr\u003e\nGene ID: 14964\u003cbr\u003e\nUniProt: View information about H-2Dk on UniProt.org\u003cbr\u003e\nClone: 15-5-5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MHC class I\u003cbr\u003e\nIsotype: Mouse (C3H.SW) IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860811010217,"sku":"110307","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-110307","provider":"Iright","version":"1.0","type":"link"}