{"product_id":"biolegend-110718","title":"Biolegend, 110718, Alexa Fluor® 488 anti-mouse CD45.1 Antibody, 100μg","description":"\u003cp\u003eCD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL\/J, STS\/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: SJL mouse thymocytes and splenocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) Shen FW, et al. 1986. Immunogenetics 24:146. (IP) Suzuki K, et al. 2000. Immunity 13:691. (IHC-F) Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F) Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F) Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F) Pascal V, et al. 2007. J. Immunol. 179:1751. (FC) Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC) Phan TG, et al. 2007. Nature Immunol. 8:992. (FC) Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed Pascal V, et al.2007. J. Immunol. 179:1751. PubMed Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed Li LX, et al. 2010. J. Immunol. 184:1728. PubMed Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed Kenna TJ, et al. 2008. Blood 111:2091. PubMed Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed\u003cbr\u003e\nProduct Citations: Whyte CE, et al. 2022. Curr Protoc. 2:e589. PubMed Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed Enterina JR, et al. 2022. Cell Rep. 38:110512. PubMed Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed Alam J, et al. 2022. Front Med (Lausanne). 9:849990. PubMed Gerber AN, et al. 2021. Cell Rep. 37:109816. PubMed Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed Fumagalli V, et al. 2020. J Exp Med. :217. PubMed El Amki M, et al. 2020. Cell Rep. 33:108260. PubMed Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed Sharma S, et al. 2015. J Immunol. 194:5529. PubMed Emgård J, et al. 2018. Immunity. 143:419. PubMed Chopin M et al. 2018. Immunity. 50(1):77-90 . PubMed Alam J, et al. 2021. Front Immunol. 12:701415. PubMed Madenspacher JH, et al. 2020. JCI Insight. 5:00. PubMed Wu T, et al. 2014. J Leukoc Biol. 95:215. PubMed Pasciuto E, et al. 2020. Cell. 182:625. PubMed Tang Q, et al. 2021. Immunity. 54:1961. PubMed Hirasawa M, et al. 2016. J Biol Chem. 291: 7373-7385. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Allman W, et al. 2015. Proc Natl Acad Sci U S A. 112: 4094 - 4103. PubMed\u003cbr\u003e\nRRID: AB_492863 (BioLegend Cat. No. 110717) AB_492862 (BioLegend Cat. No. 110718)\u003cbr\u003e\nStructure: Protein tyrosine phosphatase (PTP) family, 180-240 kD\u003cbr\u003e\nDistribution: All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice\u003cbr\u003e\nFunction: Phosphatase, T and B cell activation\u003cbr\u003e\nLigand\/Receptor: Galectin-1, CD2, CD3, CD4\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851.\u003cbr\u003e\nGene ID: 19264\u003cbr\u003e\nUniProt: View information about CD45.1 on UniProt.org\u003cbr\u003e\nClone: A20\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T200, Ly-5.1, LCA\u003cbr\u003e\nIsotype: Mouse (A.SW) IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862519664809,"sku":"110718","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-110718","provider":"Iright","version":"1.0","type":"link"}