{"product_id":"biolegend-111705","title":"Biolegend, 111705, PerCP\/Fire™ 780 anti-mouse F4\/80 Antibody, 25μg","description":"\u003cp\u003eF4\/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4\/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4\/80 has also been shown on a subset of dendritic cells. The biological ligand of F4\/80 has not been identified, but it has been reported that F4\/80 is required for induction of CD8 + T cells-mediated peripheral tolerance.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Fire™ 780 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP\/Fire™ 780 has a maximum excitation of 478 nm and a maximum emission of 780 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nRRID: AB_3662171 (BioLegend Cat. No. 111705) AB_3662171 (BioLegend Cat. No. 111706)\u003cbr\u003e\nStructure: EGF-TM7 family member, 160 kD glycoprotein\u003cbr\u003e\nDistribution: Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells\u003cbr\u003e\nFunction: Induction of immunological tolerance\u003cbr\u003e\nCell Type: Macrophages, Monocytes\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nAntigen References: Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805. Hume DA, et al. 1983. J. Exp. Med. 158:1522. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486. Lin HH, et al. 2005. J. Exp. Med. 201:1615.\u003cbr\u003e\nGene ID: 13733\u003cbr\u003e\nUniProt: View information about F4\/80 on UniProt.org\u003cbr\u003e\nClone: W20065D\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: EMR1, Ly71\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866419941545,"sku":"111705","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-111705","provider":"Iright","version":"1.0","type":"link"}