{"product_id":"biolegend-113005","title":"Biolegend, 113005, APC anti-mouse CD120a (TNF R Type I\/p55) Antibody, 25μg","description":"\u003cp\u003eCD120a is a 55 kD Type I transmembrane protein, also known as Tumor Necrosis Factor Receptor Type I (TNFRI) or p55. It is expressed on a variety of cells at low levels. Resting T cells and erythrocytes express very little to no CD120a. This receptor binds both TNF-α and LT-α (also known as TNF-β). In association with TRADD and RIP, the receptor crosslinking induced by TNF-α or LT-α trimers is critical for signal transduction, leading to apoptosis, NF-kB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: E. coli-expressed extracellular domain of the mouse TNFRI protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Flow Cytometry3,4: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 113003\/113004)or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)). ELISA Capture2: The 55R-286 antibody is useful as a capture antibody when paired with the biotinylated 55R-170 antibody (Cat. No. 112904) as the detecting antibody. Additional reported applications (for the relevant formats) include: Western blotting and immunoprecipitation1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Pinckard JK, et al. 1997. J. Biol Chem. 272:10784. (IP)Sheehan KC, et al. 1995. J. Exp. Med. 181:607. (ELISA Capture)Schlaepfer DD, et al. 2007. J. Biol. Chem. 282:17450. (FC)Bell JH, et al. 2007. J. Leukocyte Biol. 82:173. (FC)Kuroishi T, et al. 2008. J. Leukocyte Biol. 83:912.de Oliveria DC, et al. 2014. Cytokine. 69:218. PubMedKumari S, et al. 2014. Elife. 3:3422. PubMed\u003cbr\u003e\nProduct Citations: Adelaja A, et al. 2021. Immunity. 54(5):916-930.e7. PubMed Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed Jiang Y, et al. 2019. J Immunother Cancer. 7:28. PubMed Xiao Z, et al. 2022. Cell Death Dis. 13:885. PubMed Akagi T, et al. 2022. Front Immunol. 13:926175. PubMed Zhu XG, et al. 2020. Cell Metabolism. 33(1):211-221.e6. PubMed Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed Kumari S, et al. 2014. Elife. 3:3422. PubMed Dudeck J, et al. 2021. Immunity. 54(3):468-483.e5. PubMed Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed Oliveira D, et al. 2014. Cytokine. 69:218. PubMed Desu HL, et al. 2021. Cells. 10:. PubMed\u003cbr\u003e\nRRID: AB_2208780 (BioLegend Cat. No. 113005) AB_2208779 (BioLegend Cat. No. 113006)\u003cbr\u003e\nStructure: TNFR superfamily, 55 kD\u003cbr\u003e\nDistribution: Variety of cell types at low levels\u003cbr\u003e\nFunction: Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation\u003cbr\u003e\nLigand\/Receptor: TNF-α, LT-α (TNF-β)\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Aggarwal BB, et al. 1985. Nature 318 665. 2. Chan FKM, et al. 2000. Science 288:2351. 3. Loetscher H, et al. 1990. Cell 61:351. 4. Rothe J, et al. 1993. Nature 364:798.\u003cbr\u003e\nGene ID: 21937\u003cbr\u003e\nUniProt: View information about CD120a on UniProt.org\u003cbr\u003e\nClone: 55R-286\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: TNFR type I, TNFRI, TNFR60, p55, TNFRSF1A\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862444036265,"sku":"113005","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-113005","provider":"Iright","version":"1.0","type":"link"}