{"product_id":"biolegend-114406","title":"Biolegend, 114406, FITC anti-mouse I-A, 500μg","description":"\u003cp\u003eThe 25-9-17 antibody reacts with the beta chain of the I-A b MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 b bearing mice and are involved in antigen presentation to T cells expressing CD3\/TCR and CD4 proteins. The 25-9-17 antibody cross-reacts with alloantigens of the H-2 d, p, q haplotypes.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: C3H.SW splenocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity3, immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections4,5 and acetone fixed epidermal sheets6.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Wall KA, et al. 1983. J. Immunol. 131:1056. (IP) Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747. Ozato K, et al. 1981. J. Immunol. 126:317. Khanna A, et al. 2000. J. Immunol. 164:1346. (IHC) Nikolic B, et al. 1999. J. Immunol. 162:3402. (IHC) Meuter S, et al. 2007. Mol. Cell. Biol. 27:983. (IHC) PubMed Ryan AO, et al. 2011. J Exp Med. 208;1041. PubMed. Sicking EM, et al. 2012. J Am Soc Nephrol. 23:629. PubMed.\u003cbr\u003e\nProduct Citations: Chakrabarti J, et al. 2018. Oncotarget. 9:37439. PubMed Calcinotto A, et al. 2018. Nat Commun. 9:4832. PubMed Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed\u003cbr\u003e\nRRID: AB_313581 (BioLegend Cat. No. 114406)\u003cbr\u003e\nStructure: ß chain of I-Ab MHC class II\u003cbr\u003e\nDistribution: B cell and activated T cells, APCs of H-2b mice\u003cbr\u003e\nFunction: Antigen presentation\u003cbr\u003e\nLigand\/Receptor: CD3\/TCR, CD4\u003cbr\u003e\nCell Type: Antigen-presenting cells, B cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.\u003cbr\u003e\nGene ID: 14961\u003cbr\u003e\nUniProt: View information about I-Ab on UniProt.org\u003cbr\u003e\nClone: 25-9-17\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MHC class II\u003cbr\u003e\nIsotype: Mouse (C3H) IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860879626409,"sku":"114406","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-114406","provider":"Iright","version":"1.0","type":"link"}