{"product_id":"biolegend-114501","title":"Biolegend, 114501, Purified anti-mouse H-2L, 50μg","description":"\u003cp\u003eThe 28-14-8 antibody reacts with the H-2D b MHC class I alloantigen expressed on nucleated cells from mice of the H-2D b haplotype. H-2D b is involved in antigen presentation to T cells expressing CD3\/TCR and CD8 proteins. The 28-14-8 antibody has been reported to block antigen-specific lysis of target cells by cytotoxic T lymphocytes. The 28-14-8 antibody cross-reacts with the H-2L d haplotype. Clone 28-14-8 recognizes the α3 domain of H-2D b .\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: C3H.SW mouse splenocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IHC-F, IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation3, blocking of cell lysis, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 114510). Does not react with other haplotypes (e.g., k, f, p, r, s).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ozato K, et al. 1980. J. Immunol. 125:2473. Kafrouni MI, et al. 2001. J. Immunol. 167:1566. Marguet D, et al. 1999. Immunity 11:231. Bashuda H, et al. 1997. Transplantation 63:113. Greeneltch KM, et al. 2007. Cancer Res.67:10406. PubMed Milstein O, et al. 2011. Blood 117:1042.\u003cbr\u003e\nProduct Citations: Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed Azuma T, et al. 2008. Blood. 111:3635. PubMed Frey BF, et al. 2018. J Immunol. 200:1853. PubMed Cheung KCP, et al. 2020. Nat Commun. 2.954861111. PubMed\u003cbr\u003e\nRRID: AB_313583 (BioLegend Cat. No. 114502)\u003cbr\u003e\nStructure: MHC class I\u003cbr\u003e\nDistribution: Nucleated cells of the H-2Ld\/H-2Db haplotype\u003cbr\u003e\nFunction: Antigen presentation\u003cbr\u003e\nLigand\/Receptor: CD3\/TCR, CD8\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. 3. York IA, et al. 1996. Ann. Rev. Immunol. 14:369.\u003cbr\u003e\nGene ID: 1496414972\u003cbr\u003e\nUniProt: View information about H-2Ld\/H-2Db on UniProt.org\u003cbr\u003e\nClone: 28-14-8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MHC class I\u003cbr\u003e\nIsotype: Mouse (C3H\/HeJ) IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860882477225,"sku":"114501","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-114501","provider":"Iright","version":"1.0","type":"link"}