{"product_id":"biolegend-114606","title":"Biolegend, 114606, FITC anti-mouse H-2K, 500μg","description":"\u003cp\u003eThe 28-8-6 antibody reacts with the H-2K b and H-2D b MHC class I alloantigens expressed on nucleated cells from mice of the H-2K b \/H-2D b haplotype. H-2K b \/H-2D b is involved in antigen presentation to T cells expressing CD3\/TCR and CD8 proteins. The 28-8-6 antibody cross-reacts with H-2D d MHC class I alloantigen, but does not react with alloantigens of f, k, p, q, r, s haplotypes.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: C3H.SW mouse splenocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1, and immunohistochemical staining2 of acetone-fixed frozen sections.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ozato K, et al. 1981. J. Immunol. 126:317. (Cyt) Pappo J, et al. 1999. Infect. Immun. 67:337. (IHC) Bui JD, et al.2006. J. Immunol. 176:905. (FC) PubMed. Shao H, et al. 2005. J. Immunol. 175:1851. (FC)\u003cbr\u003e\nProduct Citations: Ji Y, et al. 2023. Nat Cell Biol. 25:726. PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Rezende R, et al. 2015. Nat Commun. 6: 8726. PubMed Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed Bruna A, et al. 2016. Cell. 167: 260-274. PubMed Hu HJ, et al. 2020. Cell Death Dis. 1.168055556. PubMed Liu X, et al. 2020. Nature. . PubMed Lu SX, et al. 2021. Cell. . PubMed He X, et al. 2009. Stem Cells. 27:1487. PubMed Gross ETE, et al. 2018. Oncoimmunology. 8:e1404212. PubMed Shen M, et al. 2022. Nat Cancer. 3:60. PubMed Yang Z, et al. 2021. Nat Commun. 12:4852. PubMed Taylor D, et al. 2022. Front Immunol. 13:936129. PubMed Saveljeva S, et al. 2022. Cell Metab. 34:106. PubMed Kang YH, et al. 2019. Nat Commun. 10:912. PubMed Ueda N, et al. 2006. Int Immunol. 1.720138889. PubMed Ng S, et al. 2022. Sci Rep. 12:14087. PubMed Nakagawa Y, et al. 2015. Immunol Lett. 167: 72-86. PubMed Tran NL, et al. 2022. Elife. 11:. PubMed Malenica I, et al. 2021. Nat Commun. 12:5209. PubMed Bui J, et al. 2006. J Immunol. 176:905. PubMed\u003cbr\u003e\nRRID: AB_313596 (BioLegend Cat. No. 114605) AB_313597 (BioLegend Cat. No. 114606)\u003cbr\u003e\nStructure: MHC class I\u003cbr\u003e\nDistribution: Nucleated cells of the H-2Kb\/H-2Db haplotype\u003cbr\u003e\nFunction: Antigen presentation\u003cbr\u003e\nLigand\/Receptor: CD3\/TCR, CD8\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Ozato K, et al. 1981. J. Immunol. 126:317. 2. Allen H, et al. 1986. P. Natl. Acad. Sci. USA 83:7447. 3. Evans GA, et al. 1992. Nature 300:755.\u003cbr\u003e\nGene ID: 1496414972\u003cbr\u003e\nUniProt: View information about H-2Kb\/H-2Db on UniProt.org\u003cbr\u003e\nClone: 28-8-6\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MHC class I\u003cbr\u003e\nIsotype: Mouse (C3H) IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860882673833,"sku":"114606","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-114606","provider":"Iright","version":"1.0","type":"link"}