{"product_id":"biolegend-114906","title":"Biolegend, 114906, FITC anti-mouse H-2K, 500μg","description":"\u003cp\u003eThe 36-7-5 antibody reacts with the H-2K k MHC class I alloantigen expressed on nucleated cells from mice of the H-2K k haplotype. H-2K k is involved in antigen presentation to T cells expressing CD3\/TCR and CD8 proteins. The 36-7-5 antibody does not react with alloantigens form other haplotypes (b, d, q), but weakly cross-reacts with splenocytes from SJL\/Hsd mice.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: A.AL mouse splenocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity, and immunohistochemistry2,3 of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 114914). Does not react with alloantigens of other haplotypes (e.g., b, d, q).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Goudeau B, et al. 2003. P. Natl. Acad. Sci. USA 100:15800. Johnson RM. 2004. Infect. Immun. 72:3951. Ardehali A, et al. 1995. Circulation 92:450.\u003cbr\u003e\nProduct Citations: Day E, et al. 2011. J Immunol. 186:6319. PubMed Ishikura N, et al. 2022. Oncol Rep. 47:. PubMed\u003cbr\u003e\nRRID: AB_313612 (BioLegend Cat. No. 114905)\u003cbr\u003e\nStructure: MHC class I\u003cbr\u003e\nDistribution: Nucleated cells of the H-2kk haplotype\u003cbr\u003e\nFunction: Antigen presentation\u003cbr\u003e\nLigand\/Receptor: CD3\/TCR, CD8\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. 3. Ian A, et al. 1996. Ann. Rev. Immunol. 14:369.\u003cbr\u003e\nGene ID: 14972\u003cbr\u003e\nUniProt: View information about H-2Kk on UniProt.org\u003cbr\u003e\nClone: 36-7-5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MHC class I\u003cbr\u003e\nIsotype: Mouse (A.TL) IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862380040361,"sku":"114906","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-114906","provider":"Iright","version":"1.0","type":"link"}