{"product_id":"biolegend-115001","title":"Biolegend, 115001, Purified anti-mouse I-A, 50μg","description":"\u003cp\u003eThe 39-10-8 antibody reacts with the I-A d MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 d bearing mice and are involved in antigen presentation to T cells expressing CD3\/TCR and CD4 proteins. The 39-10-8 antibody does not cross-react with other haplotypes (a, b, k, p, q, s), but has been demonstrated to cross-react with the g7 haplotype.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: (C3H x BALB\/c)F1 mouse cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IHC-F - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, and immunohistochemical staining of acetone-fixed frozen sections. Does not cross-react with other haplotypes (e.g., a, b, k, p, q, s).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Hiramine C, et al. 1995. Cell. Immunol. 160:157. Wang Z, et al. 2004. J. Immunol. 172:5924. Ma XT, et al. 2006. Cancer Research 66:1169. Norian LA and Allen PM. 2004. J. Immunol. 173:835. PubMed Tian C, et al. 2007. J. Immunol. 179:6762.\u003cbr\u003e\nProduct Citations: Ebrahimi-Nik H, et al. 2021. Nat Commun. 12:6423. PubMed Allen L 2004. J Immunol. 173:835. PubMed\u003cbr\u003e\nRRID: AB_313617 (BioLegend Cat. No. 115002)\u003cbr\u003e\nStructure: MHC class II\u003cbr\u003e\nDistribution: B cell and activated T cells, APCs of H-2d mice\u003cbr\u003e\nFunction: Antigen presentation\u003cbr\u003e\nLigand\/Receptor: CD3\/TCR, CD4\u003cbr\u003e\nCell Type: Antigen-presenting cells, B cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. 3. Wall KA, et al. 1983. J. Immunol. 131:1056. 4. Ridgway WM, et al. 1998. J. Exp. Med. 188:2267.\u003cbr\u003e\nGene ID: 14961\u003cbr\u003e\nUniProt: View information about I-Ad on UniProt.org\u003cbr\u003e\nClone: 39-10-8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MHC class II\u003cbr\u003e\nIsotype: Mouse (C3H.SW) IgG3, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862382629033,"sku":"115001","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-115001","provider":"Iright","version":"1.0","type":"link"}