{"product_id":"biolegend-115531","title":"Biolegend, 115531, PerCP anti-mouse CD19 Antibody, 25μg","description":"\u003cp\u003eCD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse CD19-expressing K562 human erythroleukemia cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunofluorescence7.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Shoham T, et al. 2003. J. Immunol. 171:4062. (FC) Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC) Kamimura D, et al. 2006. J. Immunol. 177:306. (FC) Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC) Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC) Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC) Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed Olive V, et al. 2013. Elife. 2:822. PubMed Miyai T, et al. 2014. PNAS. 111:11780. PubMed\u003cbr\u003e\nProduct Citations: Quah B, Parish C 2012. J Immunol Methods. 379:1. PubMed Sheng YH, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:789. PubMed Ajay AK, et al. 2022. Cell Rep. 38:110473. PubMed Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed Miyai T, et al. 2014. Proc Natl Acad Sci U S A. 111:1780. PubMed Han J, et al. 2018. BMC Res Notes. 11:229. PubMed Demircioglu F, et al. 2020. Nat Commun. 11:1290. PubMed Luo Z, et al. 2020. Front Immunol. 0.883333333. PubMed Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed Ylösmäki E, et al. 2021. Mol Ther Oncolytics. 459:20. PubMed Ylösmäki E, et al. 2021. J Immunother Cancer. 9:. PubMed Dutow P, et al. 2014. J Infect Dis. 209:1269. PubMed Liu S, et al. 2020. Cell Host \u0026amp; Microbe. 26(6):779-794.e8.. PubMed Fusciello M, et al. 2022. Mol Ther Oncolytics. 25:137. PubMed Murdock BJ, et al. 2021. JCI Insight. 6:. PubMed Jesus M, et al. 2013. J Vis Exp. 73: 50167. PubMed Bergquist M, et al. 2014. J Infect. 69:113. PubMed Lang JF et al. 2019. Nat Commun. 10(1):3415 . PubMed Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed\u003cbr\u003e\nRRID: AB_893278 (BioLegend Cat. No. 115531) AB_2072926 (BioLegend Cat. No. 115532)\u003cbr\u003e\nStructure: Ig superfamily, associates with CD21 and CD81, 95 kD\u003cbr\u003e\nDistribution: Pro-B cells to mature B cells (during development), follicular dendritic cells\u003cbr\u003e\nFunction: Modulates B cell activation and differentiation\u003cbr\u003e\nLigand\/Receptor: CD21, CD81, Leu-13\u003cbr\u003e\nCell Type: B cells, Dendritic cells\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341. 2. Krop I, et al. 1996. Eur. J. Immunol. 26:238. 3. Krop I, et al. 1996. J. Immunol. 157:48. 4. Tedder TF, et al. 1994. Immunol. Today 15:437.\u003cbr\u003e\nGene ID: 12478\u003cbr\u003e\nUniProt: View information about CD19 on UniProt.org\u003cbr\u003e\nClone: 6D5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: B4\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862476804265,"sku":"115531","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-115531","provider":"Iright","version":"1.0","type":"link"}