{"product_id":"biolegend-116407","title":"Biolegend, 116407, PE anti-mouse I-A, 50μg","description":"\u003cp\u003eThe AF6-120.1 antibody reacts with the I-A b MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 b bearing mice, and are involved in antigen presentation to T cells expressing CD3\/TCR and CD4 proteins. The AF6-120.1 antibody cross-reacts with H-2 k and H-2 u haplotypes; this antibody does not cross-react with other haplotypes (d, f, q, r, s).\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: C57BL\/10J splenocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of frozen sections (acetone-fixed5; OCT-embedded, ethanol-fixed sections7), immunofluorescence microscopy3 (including acetone-fixed epidermal sheets6), immunoprecipitation7,8. Directly conjugated antibody was used for IF in (3) and (6) and for IHC in (5). Does not react with other haplotypes (e.g., d, f, q, r, s).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Wall KA, et al. 1983. J. Immunol. 131:1056. (FC) Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747. (FC) Inaba K, et al. 1998. J. Exp. Med. 188:2163 (IF) Hamrah P, et al. 2002. Invest Opthalmol Vis. Sci. 43:639 (IF) Buono C, et al. 2003. Arterioscler. Thromb. Vasc. Biol. 23:454. (IHC) Wang Z, et al. 2004. J. Immunol. 172:5924. (IHC IF) Nakagawa TY, et al. 1999. Immunity 10:207. (IP) Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC IP) PubMed Schneppenheim J, et al. 2013. J Exp Med. 210:41. PubMed.\u003cbr\u003e\nProduct Citations: Sang H, et al. 2023. Adv Clin Exp Med. 32:551. PubMed Ishida K, et al. 2023. Front Immunol. 14:1111729. PubMed Beuneu H, et al. 2006. J Immunol . 177:1406. PubMed Wu J, et al. 2021. Mol Med Rep. 23:. PubMed Liang Y, et al. 2014. J Immunol. 192:1277. PubMed Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed Zhou J, et al. 2022. Int J Oncol. 61: . PubMed Zager A, et al. 2017. Progress in Neuro-Psychopharmacology and Biological Psychiatry. 10.1016\/j.pnpbp.2017.05.003. PubMed Song S, et al. 2021. Front Immunol. 12:705140. PubMed Su M, et al. 2018. Immunol Cell Biol. 9:2448. PubMed\u003cbr\u003e\nRRID: AB_313726 (BioLegend Cat. No. 116407) AB_313727 (BioLegend Cat. No. 116408)\u003cbr\u003e\nStructure: MHC class II\u003cbr\u003e\nDistribution: B cell and activated T cells, APCs of H-2b mice\u003cbr\u003e\nFunction: Antigen presentation\u003cbr\u003e\nLigand\/Receptor: CD3\/TCR, CD4\u003cbr\u003e\nCell Type: Antigen-presenting cells, B cells, T cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Watts C. 1997. Annu. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323.\u003cbr\u003e\nGene ID: 14961\u003cbr\u003e\nUniProt: View information about I-Ab on UniProt.org\u003cbr\u003e\nClone: AF6-120.1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MHC class II\u003cbr\u003e\nIsotype: Mouse (BALB\/c) IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862534082729,"sku":"116407","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-116407","provider":"Iright","version":"1.0","type":"link"}