{"product_id":"biolegend-120306","title":"Biolegend, 120306, PE anti-mouse GITR Ligand Antibody, 100μg","description":"\u003cp\u003eGITR ligand (GITRL) is a member of the TNF superfamily, also known as TNFSF18 or TL6. It is expressed on B cells, macrophages, dendritic cells and endothelial cells. The expression of GITRL on dendritic cell surface can be transiently up-regulated and then rapidly down-regulated by LPS stimulation. GITRL binds to its receptor, GITR, to co-stimulate T cell activation, proliferation, and cytokine production, and inhibit the suppressive functions of CD4 + CD25 + Treg cells, as well as induce macrophages activation. GITR-GITRL interaction has been implicated as an essential regulator of immune response to self antigens and phathogens.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant mouse GITRL protein and Transfected cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059Lu LF, et al. 2005. J. Immunol. 175:1651Esparza EM and RH Arch. 2005. J. Immunol. 174:7875Ray A, et al. 2012. J. Immunol. 188:3188. PubMed.\u003cbr\u003e\nProduct Citations: Li H, et al. 2022. Cancer Cell. 40:36. PubMed Hinks TSC, et al. 2019. Cell Rep. 28:3249. PubMed Ray A, et al. 2012. J Immunol. 188:3188. PubMed Zhang M, et al. 2017. Mol Med Rep. 15:4291. PubMed\u003cbr\u003e\nRRID: AB_2287690 (BioLegend Cat. No. 120305) AB_2207248 (BioLegend Cat. No. 120306)\u003cbr\u003e\nStructure: TNF superfamily member\u003cbr\u003e\nDistribution: B cells, macrophages, dendritic cells, endothelial cells.\u003cbr\u003e\nFunction: Bind GITR receptor to co-stimulate T cells activation, proliferation, and cytokine production; inhibit the suppressive activities of CD4+CD25+ Treg cells.\u003cbr\u003e\nLigand\/Receptor: GITR (TNFRSF18)\u003cbr\u003e\nCell Type: B cells, Macrophages, Dendritic cells, Endothelial cells, Tregs\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nAntigen References: 1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059. 2. Kim JD, et al. 2003. Genes Immun. 4:564. 3. Yu KY, et al. 2003. Biochem. Biophys. Res. Commun. 310:433. 4. Shin HH, et al. 2002. Cytokine 19:187.\u003cbr\u003e\nGene ID: 240873\u003cbr\u003e\nUniProt: View information about GITRL on UniProt.org\u003cbr\u003e\nClone: YGL 386\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: AITRL, TNFSF18, TL6, GITRL\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864105767081,"sku":"120306","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-120306","provider":"Iright","version":"1.0","type":"link"}