{"product_id":"biolegend-121917","title":"Biolegend, 121917, PerCP\/Cyanine5.5 anti-mouse CD309 (VEGFR2, Flk-1) Antibody, 25μg","description":"\u003cp\u003eThe 89B3A5 antibody recognizes mouse CD309 also known as vascular endothelial growth factor receptor 2, VEGFR2, KDR, protein tyrosine kinase receptor flk-1, and fetal liver kinase-1. Flk-1 is a member of the tyrosine protein kinase family, sub-family CSF-1\/PDGF, that contains a single pass transmembrane receptor with a protein kinase domain and seven immunoglobulin-like domains in the extracellular region. Flk-1 is expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle; other tissues express at lower levels. Flk-1 is a receptor for VEGF or VEGFC; ligand binding plays a key role in vascular development and vascular permeability. The 89B3A5 antibody has been shown to be useful for flow cytometry.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Rat-1 cells transfected with full-length mouse Flk\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kaburn N, et al. 1997. Development 124:2039.\u003cbr\u003e\nProduct Citations: Wang C, et al. 2021. Cell Rep. 37:110021. PubMed Harbuzariu A, et al. 2014. J Vasc Surg. 59:1686. PubMed Farren M, et al. 2014. Sci Signal. 18:313. PubMed\u003cbr\u003e\nRRID: AB_893649 (BioLegend Cat. No. 121917) AB_893646 (BioLegend Cat. No. 121918)\u003cbr\u003e\nStructure: Member of the tyrosine protein kinase family, sub-family CSF-1\/PDGF. Single pass transmembrane receptor containing a protein kinase domain and seven immunoglobulin-like domains.\u003cbr\u003e\nDistribution: Expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle; other tissues express at lower levels.\u003cbr\u003e\nFunction: Receptor for VEGF or VEGFC. Ligand binding plays a key role in vascular development and vascular permeability.\u003cbr\u003e\nLigand\/Receptor: VEGF, VEGFC\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Biology, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Patterson C, et al. 1995. J. Biol. Chem. 270:23111. 2. Quinn TP, et al. 1993. Proc. Natl. Acad. Sci. USA 90:7533.\u003cbr\u003e\nGene ID: 16542\u003cbr\u003e\nUniProt: View information about CD309 on UniProt.org\u003cbr\u003e\nClone: 89B3A5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Vascular endothelial growth factor receptor 2, VEGFR2, KDR, protein tyrosine kinase receptor flk-1, fetal liver kinase-1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862492369065,"sku":"121917","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-121917","provider":"Iright","version":"1.0","type":"link"}