{"product_id":"biolegend-122502","title":"Biolegend, 122502, Purified anti-mouse Ly-6A\/E (Sca-1) Antibody, 500μg","description":"\u003cp\u003eLy-6A\/E is an 18 kD member of the Ly-6 multigene family also known as Sca-1. Ly6A\/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA\/2, SJL, SWR, and 129), Ly-6A\/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB\/c, CBA, C3H\/He, DBA\/1, and NZB) have low Ly-6A\/E expression on resting peripheral lymphocytes. The expression of Ly-6A\/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A\/E is thought to be involved in the regulation of both T and B cell responses. The E13-161.7 antibody has been reported to be useful for immunohistochemical staining of frozen sections, immunoprecipitation, flow cytometry, and complement-mediated cytotoxicity.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: mouse pre-T cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality testedIHC-F, IHC-P, IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1, immunohistochemical staining of frozen sections2 and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.7\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD) Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC) van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP) van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF) Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294. Artinger EL, et al. 2013. PNAS. 110:12000. PubMed\u003cbr\u003e\nProduct Citations: Chartrand K, et al. 2018. Front Immunol. 1.642361111. PubMed Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed Goldsmith JR, et al. 2020. Nat Commun. 11:2591. PubMed Lee JH, et al. 2022. iScience. 25:104166. PubMed Gronowicz G, et al. 2017. Tissue Eng Part A. 23:1490. PubMed Yao L, et al. 2021. J Bone Miner Res. 36:1159. PubMed Felthaus O, et al. 2010. Neurosci Lett. 471:179. PubMed Brandl C, et al. 2009. Exp Eye Res. 89:209. PubMed Martin K,et al. 2017. J Immunol.. 10.4049\/jimmunol.1601522. PubMed Umemoto T, et al. 2017. EMBO J. 36:2390. PubMed Shah K, et al. 2022. Nat Commun. 13:1827. PubMed Nascimento DC, et al. 2017. Nat Commun. 8:14919. PubMed Hsieh HY, et al. 2020. Cancer Sci. 111:2400. PubMed Helbling PM, et al. 2019. Cell Rep. 29:3313. PubMed Ishii H, et al. 2018. Nat Commun. 2.188194444. PubMed Rogers P, Driessnack M, and Schwartz E. 2017. PLoS One. 10.1371\/journal.pone.0181985. PubMed Zhang C, et al. 2018. Cell Res. 28:323. PubMed Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed\u003cbr\u003e\nRRID: AB_756186 (BioLegend Cat. No. 122501) AB_756187 (BioLegend Cat. No. 122502)\u003cbr\u003e\nStructure: Ly-6 multigene family, 18 kD\u003cbr\u003e\nDistribution: Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells\u003cbr\u003e\nFunction: Regulates B and T cell responses\u003cbr\u003e\nCell Type: B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells\u003cbr\u003e\nBiology Area: Immunology, Stem Cells\u003cbr\u003e\nAntigen References: 1. Rock KL, et al. 1989. Immunol. Rev. 111:195. 2. Morrison SJ, et al. 1994. Immunity 1:661. 3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. 4. Malek T, et al. 1986. J. Exp. Med. 164:709.\u003cbr\u003e\nGene ID: 110454\u003cbr\u003e\nUniProt: View information about Ly-6A\/E on UniProt.org\u003cbr\u003e\nClone: E13-161.7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Sca-1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866087379113,"sku":"122502","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-122502","provider":"Iright","version":"1.0","type":"link"}