{"product_id":"biolegend-122513","title":"Biolegend, 122513, PE\/Cyanine7 anti-mouse Ly-6A\/E (Sca-1) Antibody, 25μg","description":"\u003cp\u003eLy-6A\/E is an 18 kD member of the Ly-6 multigene family also known as Sca-1. Ly6A\/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA\/2, SJL, SWR, and 129), Ly-6A\/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB\/c, CBA, C3H\/He, DBA\/1, and NZB) have low Ly-6A\/E expression on resting peripheral lymphocytes. The expression of Ly-6A\/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A\/E is thought to be involved in the regulation of both T and B cell responses. The E13-161.7 antibody has been reported to be useful for immunohistochemical staining of frozen sections, immunoprecipitation, flow cytometry, and complement-mediated cytotoxicity.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: mouse pre-T cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1, immunohistochemical staining of frozen sections2 and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.7\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD) Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC) van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP) van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF) Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294. Artinger EL, et al. 2013. PNAS. 110:12000. PubMed\u003cbr\u003e\nProduct Citations: Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed Rahman SMT, et al. 2022. Cell Rep. 41:111682. PubMed Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed Levra Levron C, et al. 2023. Nat Cell Biol. 25:740. PubMed Zachara M, et al. 2022. EMBO J. 41:e108206. PubMed Ramdas B, et al. 2022. Mol Ther. 30:2505. PubMed Li X, et al. 2022. Cell. 185:1709. PubMed Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed Mitroulis I et al. 2018. Cell. 172(1-2):147-161 . PubMed Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed Guo B, et al. 2018. Nat Med. 24:360. PubMed Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed Hara T, et al. 2012. J Immunol. 189:1577. PubMed Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed Jia W, et al. 2021. Nat Commun. 12:2118. PubMed Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed Bues J, et al. 2022. Nat Methods. 19:323. PubMed Cheng Y, et al. 2019. Cell Rep. 28:1703. PubMed Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed Fournier M, Bijl C 2015. Stem Cells Dev. 24: 2413-2422. PubMed Sztwiertnia I, et al. 2020. PLoS One. 15:e0233789. PubMed Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed Kalafati L, et al. 2020. Cell. 183:771. PubMed Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed Thürmer M, et al. 2022. Nat Commun. 13:2982. PubMed Lefkopoulos S, et al. 2020. Immunity. 53(5):934-951.e9. PubMed Muzumdar S, et al. 2019. Am J Pathol. 189:568. PubMed Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed Luo H, et al. 2019. Cell Rep. 26:945. PubMed\u003cbr\u003e\nRRID: AB_756198 (BioLegend Cat. No. 122513) AB_756199 (BioLegend Cat. No. 122514)\u003cbr\u003e\nStructure: Ly-6 multigene family, 18 kD\u003cbr\u003e\nDistribution: Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells\u003cbr\u003e\nFunction: Regulates B and T cell responses\u003cbr\u003e\nCell Type: B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells\u003cbr\u003e\nBiology Area: Immunology, Stem Cells\u003cbr\u003e\nAntigen References: 1. Rock KL, et al. 1989. Immunol. Rev. 111:195. 2. Morrison SJ, et al. 1994. Immunity 1:661. 3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. 4. Malek T, et al. 1986. J. Exp. Med. 164:709.\u003cbr\u003e\nGene ID: 110454\u003cbr\u003e\nUniProt: View information about Ly-6A\/E on UniProt.org\u003cbr\u003e\nClone: E13-161.7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Sca-1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866087608489,"sku":"122513","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-122513","provider":"Iright","version":"1.0","type":"link"}