{"product_id":"biolegend-123105","title":"Biolegend, 123105, Biotin anti-mouse F4\/80 Antibody, 50μg","description":"\u003cp\u003eF4\/80, also known as EMR1 or Ly71, is a 160 kD glycoprotein of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4\/80 has been widely used as a murine macrophage marker. It is expressed on a majority of tissue macrophages, including macrophages in the lung, gut, peritoneal cavity, thymus, and red pulp of the spleen, Kupffer cells, Langerhans cells, microglia, and certain dendritic cells. It is not expressed on macrophages located in the T cell areas of the spleen, lymph node, or Peyer's patch. The biological ligand of F4\/80 has not been identified, but it has been reported that F4\/80 is required for the induction of CD8 + T cells-mediated peripheral tolerance.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Murine macrophages\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality testedIHC - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC) Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC) Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC) Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC) Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed Watson NB, et al. 2015. J Immunol. 194:2796. PubMed Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Sasaki O, et al. 2013. J Immunol. 191:2879. PubMed Knab L, et al. 2014. Mol Cancer Res. 12:1440. PubMed Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed McGee MC, et al. 2022. Bio Protoc. 12:. PubMed Xu H, et al. 2023. Elife. 12:. PubMed Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed Tomiaki C, et al. 2022. Front Immunol. 13:1014462. PubMed Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed Yeh CH, et al. 2022. Immunity. 55:272. PubMed Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed Boccasavia VL, et al. 2021. Cell Reports. 34(11):108861. PubMed Krueger PD, et al. 2021. Immunity. 54(4):687-701.e4. PubMed Yuan D et al. 2017. Cancer cell. 31(6):771-789 . PubMed Iturri L, et al. 2021. Immunity. . PubMed Hazama D, et al. 2020. Cell Chemical Biology. 27(9):1181-1191.e7. PubMed Joachim R, Suber F, and Kobzik L 2017. Sci Rep. . 10.1038\/s41598-017-16743-1. PubMed Georgilis A et al. 2018. Cancer cell. 34(1):85-102 . PubMed Xu S, et al. 2016. PLoS One. 11: 0163829. PubMed Wu W, et al. 2016. PLoS One. 11: 0159512. PubMed Suah AN, et al. 2021. J Clin Invest. 131:. PubMed Thion MS et al. 2018. Cell. 172(3):500-516 . PubMed Yauch L, et al. 2010. J Immunol. 185:5405. PubMed Zoller E, et al. 2011. J Exp Med. 208:1203. PubMed Goggi JL, et al. 2020. Mol Imaging Biol. 22:1392. PubMed Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed Onodera T, et al. 2019. J Immunol. 203:3282. PubMed Naing A et al. 2019. Cell reports. 26(5):1242-1257 . PubMed Linde N, et al. 2018. Nat Commun. 9:21. PubMed Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed Kim AD, et al. 2021. Sci Rep. 11:24194. PubMed Onodera T, et al. 2021. Immunity. 54:2385. PubMed Zeng Z, et al. 2022. Oncogene. :. PubMed Akk A, et al. 2019. Mol Immunol. 114:629. PubMed Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed Zeis P, et al. 2020. Immunity. 53:775. PubMed Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed Grayczyk JP et al. 2017. Cell host \u0026amp; microbe. 22(5):678-687 . PubMed Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed Matsumoto S, et al. 2010. J Immunol. 184:1543. PubMed Werner A, et al. 2021. iScience. 24:103076. PubMed Lutz J, et al. 2015. Nat Commun. 6: 8575. PubMed Calvente CJ, et al. 2019. J Clin Invest. 130:4091. PubMed Maller O, et al. 2020. Nat Mater. 20:548. PubMed Crowe J, et al. 2020. PLoS Pathog. 16:e1008391. PubMed Moriyasu T, et al. 2018. PLoS Negl Trop Dis. 12:e0006197. PubMed Liao YC, et al. 2021. Front Immunol. 12:743030. PubMed\u003cbr\u003e\nRRID: AB_893499 (BioLegend Cat. No. 123105) AB_893501 (BioLegend Cat. No. 123106)\u003cbr\u003e\nStructure: EGF-TM7 family member, 160 kD glycoprotein\u003cbr\u003e\nDistribution: Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kupffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells\u003cbr\u003e\nFunction: Induction of immunological tolerance\u003cbr\u003e\nCell Type: Dendritic cells, Langerhans cells, Macrophages, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience\u003cbr\u003e\nAntigen References: 1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805. 2. Hume DA, et al. 1983. J. Exp. Med. 158:1522. 3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801. 4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486. 5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.\u003cbr\u003e\nGene ID: 13733\u003cbr\u003e\nUniProt: View information about F4\/80 on UniProt.org\u003cbr\u003e\nClone: BM8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: EMR1, Ly71\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862912487593,"sku":"123105","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-123105","provider":"Iright","version":"1.0","type":"link"}