{"product_id":"biolegend-123133","title":"Biolegend, 123133, Brilliant Violet 605™ anti-mouse F4\/80 Antibody, 125microl","description":"\u003cp\u003eF4\/80, also known as EMR1 or Ly71, is a 160 kD glycoprotein of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4\/80 has been widely used as a murine macrophage marker. It is expressed on a majority of tissue macrophages, including macrophages in the lung, gut, peritoneal cavity, thymus, and red pulp of the spleen, Kupffer cells, Langerhans cells, microglia, and certain dendritic cells. It is not expressed on macrophages located in the T cell areas of the spleen, lymph node, or Peyer's patch. The biological ligand of F4\/80 has not been identified, but it has been reported that F4\/80 is required for the induction of CD8 + T cells-mediated peripheral tolerance.\u003cbr\u003e\n125microl\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Murine macrophages\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610\/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC) Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC) Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC) Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC) Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed Watson NB, et al. 2015. J Immunol. 194:2796. PubMed Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Gallizioli M, et al. 2020. Cell Rep. 33:108291. PubMed Schmid MC, et al. 2018. Nat Commun. 9:5379. PubMed Solier S, et al. 2023. Nature. 617:386. PubMed Vasanthakumar A, et al. 2020. Nature. 579:581. PubMed Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed Zhao K, et al. 2023. Front Immunol. 14:1101769. PubMed Li DD, et al. 2022. Cell Host Microbe. 30:530. PubMed Aguilera T, et al. 2016. Nat Commun. 7:13898. PubMed Kim YS, et al. 2021. Cell Death Dis. 12:307. PubMed Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed Vicetti Miguel R, et al. 2016. PLoS One. 11: 0162445. PubMed Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed Salas–Pérdomo A, et al. 2019. Sci Rep. 9:8309. PubMed Karachi A, et al. 2019. Neuro Oncol. 1.381944444. PubMed Cunningham C, et al. 2016. PLoS Pathog. 12: 1005356. PubMed Pandey V, et al. 2021. eLife. 0.416666666666667. PubMed Korangath P, et al. 2020. Bio Protoc. 10:e3822. PubMed Ayaub E,et al. 2017. Sci Rep.. 10.1038\/s41598-017-13511-z. PubMed Chen L, et al. 2021. Medicines (Basel). 8:. PubMed Weaver JD, et al. 2022. Oncoimmunology. 11:2141007. PubMed Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed Liu S, et al. 2020. Cell Host \u0026amp; Microbe. 26(6):779-794.e8.. PubMed Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed Ayaub EA, et al. 2019. Immunol Cell Biol. 97:203. PubMed Riding AM, et al. 2022. iScience. 25:104660. PubMed Penkert RR, et al. 2020. Obesity (Silver Spring). 1631:28. PubMed Qi F, et al. 2019. Front Microbiol. 2.140277778. PubMed Qadir AS, et al. 2021. iScience. 24:103348. PubMed Rosser EC, et al. 2020. Cell Metabolism. 31(4):837-851. PubMed Jablonski K, et al. 2015. PLoS One. 10: 0145342. PubMed Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed Shigeta A, et al. 2019. Dev Cell. 48:617. PubMed Akoolo L, et al. 2021. Cell Microbiol. 23:e13350. PubMed Aguilera TA, et al. 2020. Clin Cancer Res. 26:2972. PubMed Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed Akhand SS, et al. 2020. Cancer Immunol Res. 8:1542. PubMed Thomson CA, et al. 2018. J Immunol. 201:215. PubMed\u003cbr\u003e\nRRID: AB_2562305 (BioLegend Cat. No. 123133)\u003cbr\u003e\nStructure: EGF-TM7 family member, 160 kD glycoprotein\u003cbr\u003e\nDistribution: Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kupffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells\u003cbr\u003e\nFunction: Induction of immunological tolerance\u003cbr\u003e\nCell Type: Dendritic cells, Langerhans cells, Macrophages, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience\u003cbr\u003e\nAntigen References: 1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805. 2. Hume DA, et al. 1983. J. Exp. Med. 158:1522. 3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801. 4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486. 5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.\u003cbr\u003e\nGene ID: 13733\u003cbr\u003e\nUniProt: View information about F4\/80 on UniProt.org\u003cbr\u003e\nClone: BM8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: EMR1, Ly71\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862914453673,"sku":"123133","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-123133","provider":"Iright","version":"1.0","type":"link"}