{"product_id":"biolegend-123406","title":"Biolegend, 123406, Biotin anti-mouse CD21\/CD35 (CR2\/CR1) Antibody, 500μg","description":"\u003cp\u003eCD21, also known as CR2 (complement receptor 2) and C3d receptor, binds C3d and iC3b. It is also a receptor of Epstein-Barr virus. CD35, also known as CR1, binds C3b, iC3b, C4b, and iC4b. CD21\/CD35 is primarily expressed on B lymphocytes, mast cells, follicular dendritic cells, macrophages, and activated granulocytes. CD21\/CD35 forms part of the B-cell antigen receptor complex with CD19 and CD81 and is involved in signal transduction.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: CD35\/CFA\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Boackle S, et al. 2001 Immunity 15:775. de Andres B, et al. 2012. J. Immunol. 189:2300. PubMed Chiu YK, et al. 2014. J Immunol. 193:2207. PubMed Koening PA, et al. 2014. J Biol Chem. 289:34490. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Glaros V, et al. 2021. Immunity. 54:2005. PubMed Heesters B, et al. 2015. PLoS Pathog. 11: 1005285. PubMed Choi SY, et al. 2019. J Immunol. 203:2630. PubMed Kajihara R, et al. 2014. J Immunol. 192:5720. PubMed Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed Gibb D, et al. 2010. J Exp Med. 207:623. PubMed Rohrbeck L, et al. 2020. Immunol Cell Biol. . PubMed Wang W, et al. 2014. J Immunol. 192:3011. PubMed\u003cbr\u003e\nRRID: AB_940399 (BioLegend Cat. No. 123405) AB_940401 (BioLegend Cat. No. 123406)\u003cbr\u003e\nStructure: 145 kD\/190 kD type I transmembrane glycoprotein\u003cbr\u003e\nDistribution: B cells, follicular dendritic cells\u003cbr\u003e\nFunction: Signal transduction\u003cbr\u003e\nLigand\/Receptor: Associated with CD19\/CD81. CD21 binds C3d, iC3b, and EBV; CD35 binds C3b, iC3b, C4b, and iC4b.\u003cbr\u003e\nCell Type: B cells, Dendritic cells\u003cbr\u003e\nBiology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Kozono Y, et al. 1998. J. Immunol. 160:1562. 2. Shimizu I, et al. 2007. Blood 109:1773. 3. Roozendaal R and MC. Carroll. 2007. Immunol. Rev. 219:157.\u003cbr\u003e\nGene ID: 1290212946\u003cbr\u003e\nUniProt: View information about CD21\/CD35 on UniProt.org\u003cbr\u003e\nClone: 7E9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CR2\/CR1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862488436905,"sku":"123406","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-123406","provider":"Iright","version":"1.0","type":"link"}