{"product_id":"biolegend-124302","title":"Biolegend, 124302, Purified anti-mouse CD274 (B7-H1, PD-L1) Antibody, 500μg","description":"\u003cp\u003eCD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IHC-F - Verified Block - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunofluorescence4, blocking6,7,8,9, and immunohistochemistry of acetone-fixed frozen sections4, 11. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 124303). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124318) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin \u0026lt;0.01 EU\/µg).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Maier H, et al. 2007. J. Immunol. 178:2714. Meng Q, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:4444. PubMed Scarlett UK, et al. 2012. J Exp Med. 209:495. PubMed Grabie N, et al. 2007. Circulation 116:2062. (IF, IHC) Paterson AM, et al. 2011. J. Immunol. 187:1097. Channappanavar R, et al. 2012. PLoS One 7:e39757. (Block) Schreiber HA, et al. 2010. PLoS One 5:e11453. (Block) PubMed Muthumani K, et al. 2011. J. Immunol. 187:2932. (Block) PubMed Cripps JG, et al. 2010. Hepatology 52:1350. (Block) PubMed Murakami R, et al. 2013. PLoS One. 8:73270. PubMed Riella LV, et al. 2011. Am. J. Transplant 11:832-40. (IHC) Lei GS, et al. 2015. Infect Immun. 83:572. PubMed\u003cbr\u003e\nProduct Citations: Sun Y, et al. 2022. Mol Ther Oncolytics. 26:105. PubMed Peymanfar Y, et al. 2023. Int J Mol Sci. 24:. PubMed Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed Liu CJ, et al. 2020. Neuro Oncol. 22:1276. PubMed Li H, et al. 2022. Cancer Cell. 40:36. PubMed Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed Xiong W, et al. 2022. Nat Commun. 13:1700. PubMed Dieterich LC, et al. 2017. Front Immunol. 0.379166667. PubMed Iwai T, et al. 2021. Mol Cancer Ther. 20:2519. PubMed Muhammad F, et al. 2020. Front Immunol. 975:11. PubMed Herold M, et al. 2015. J Immunol. 195: 3584 - 3595. PubMed Wang G, et al. 2020. Nat Commun. 11:1395. PubMed Schofield DJ, et al. 2021. MAbs. 13:1857100. PubMed Tan Z, et al. 2020. Mol Ther Oncolytics. 16:302. PubMed Huang B, et al. 2015. PLoS One. 10: 0134715. PubMed Lei G, et al. 2015. Infect Immun . 83:572. PubMed Singh M, et al. 2017. Nat Commun. 8:1447. PubMed Hoves S, et al. 2018. J Exp Med. 215:859. PubMed Meng Q, et al. 2006. Invest Ophthalmol Vis Sci. 47:4444. PubMed Murakami R, et al. 2013. PLoS One. 8:73270. PubMed Yahata T, et al. 2019. Cancer Sci. 110:1279. PubMed Tanaka Y, et al. 2020. Sci Rep. 10:17284. PubMed Kong F, et al. 2021. Front Immunol. 12:670646. PubMed Speranza MC, et al. 2018. Neuro Oncol. 20:225. PubMed Muhammad F, et al. 2020. J Autoimmun. 111:102441. PubMed Scarlett U, et al. 2012. J Exp Med. 209:425. PubMed Sano Y, et al. 2021. Mol Cancer Res. 19:507. PubMed Zhao T, et al. 2022. JCI Insight. 7:. PubMed Mao W, et al. 2019. J Immunother Cancer. 0.484027778. PubMed Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed Lin A, et al. 2022. Bioeng Transl Med. 7:e10314. PubMed Bassi \u0026amp;, et al. 2012. Diabetes. 61:2534. PubMed Iida Y, et al. 2020. J Immunother Cancer. 8:. PubMed Li Z, et al. 2022. Transl Cancer Res. 11:3698. PubMed Hirose T, et al. 2017. PLoS One. 12(6):e0178765. PubMed Knier B, et al. 2018. Nat Immunol. 1.722916667. PubMed Giles DA, et al. 2018. J Clin Invest. 128:5322. PubMed Mitchell LA, et al. 2019. Oncotarget. 10:2252. PubMed\u003cbr\u003e\nRRID: AB_961226 (BioLegend Cat. No. 124301) AB_961228 (BioLegend Cat. No. 124302)\u003cbr\u003e\nStructure: 40 kD type I transmembrane protein member of B7 family within the immunoglobulin receptor superfamily\u003cbr\u003e\nDistribution: T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes\u003cbr\u003e\nLigand\/Receptor: PD-1 (PDCD1)\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Endothelial cells, Monocytes, NK cells, T cells\u003cbr\u003e\nBiology Area: Cancer Biomarkers, Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Sharpe A, et al. 2007. Nat. Immunol. 8:239. 2. Dong H, et al. 1999. Nat. Med. 5:1365. 3. Freeman G, et al. 2000. J. Exp. Med. 192:1027.\u003cbr\u003e\nGene ID: 60533\u003cbr\u003e\nUniProt: View information about CD274 on UniProt.org\u003cbr\u003e\nClone: 10F.9G2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: B7-H1, PD-L1\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860782370985,"sku":"124302","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-124302","provider":"Iright","version":"1.0","type":"link"}