{"product_id":"biolegend-124913","title":"Biolegend, 124913, APC anti-mouse DC Marker (33D1) Antibody, 25μg","description":"\u003cp\u003e33D1 antigen, also known as DCIR2 (dendritic cell inhibitory receptor 2), is reported to be a dendritic cell marker on subpopulation DC in the spleen, thymus, and Peyer's patch. It is reported that most N418 (CD11c)+ cells in spleen are also 33D1 positive. Bone marrow dendritic cells upregulate 33D1 antigen expression after incubation with GM-CSF.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse dendritic cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunofluorescence3 and immunohistochemical staining of acetone-fixed frozen sections4. Binding of clone 33D1 is calcium-dependent. We recommend staining in EDTA-free FACS buffer containing a source of Ca2+ ions.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Crowley MT, et al. 1990. J. Immunol. Methods 133:55. Nussenzwig MC, et al. 1982. P. Natl. Acad. Sci. USA 79:161. Steinman RM, et al. 1983. J. Exp. Med. 157:613. (IF) Fischer HG, et al. 2000. J. Immunol. 164:4826. (IHC)\u003cbr\u003e\nProduct Citations: Shin C, et al. 2016. Immunol Lett. 172: 21-28. PubMed\u003cbr\u003e\nRRID: AB_1227626 (BioLegend Cat. No. 124913) AB_1227625 (BioLegend Cat. No. 124914)\u003cbr\u003e\nStructure: The antigen recognized by 33D1 is yet unknown.\u003cbr\u003e\nDistribution: 33D1 antigen was reported on dendritic cell populations from mouse thymus, spleen lymph nodes and Peyer patches. Bone marrow dendritic cells can be induced to express 33D1 antigen in the presence of GM-CSF. In mouse spleen, most N418+ cells are also 33D1 positive.\u003cbr\u003e\nFunction: GM-CSF is reported to increase expression of 33D1 antigen on dendritic cells from bone marrow cells and IL-4 reported to down regulate the 33D1 antigen.\u003cbr\u003e\nCell Type: Dendritic cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about 33D1 on UniProt.org\u003cbr\u003e\nClone: 33D1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Dendritic Cell Marker, 33D1, DCIR2 (dendritic cell inhibitory receptor 2)\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862372405417,"sku":"124913","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-124913","provider":"Iright","version":"1.0","type":"link"}