{"product_id":"biolegend-125009","title":"Biolegend, 125009, Alexa Fluor® 647 anti-mouse FR4 (Folate Receptor 4) Antibody, 25μg","description":"\u003cp\u003eFolate receptor 4 (FR4) is a surface receptor for folic acid (Vitamin B9).It was recently reported that FR4 is highly and constitutively expressed on mouse CD4+ CD25+ natural regulatory T cells (Treg).The high expression of this surface marker on Treg can be used in combination with CD4 and CD25 to distinguish Treg from other types of T cells.Since FR4 is a surface marker,this antibody may be used in combination with anti-CD4 and\/or CD25 to sort living T reg cells for functional studies.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm \/ 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Yamaguchi T et al. 1007:Immunity 27(1)145\u003cbr\u003e\nProduct Citations: Inoue N, et al. 2015. Nat Commun. 6: 8858. PubMed Mori M, et al. 2021. J Cell Biol. 220:. PubMed Young A, et al. 2017. Cancer Res. 77:4684. PubMed\u003cbr\u003e\nRRID: AB_1134201 (BioLegend Cat. No. 125009)\u003cbr\u003e\nStructure: 28 kd protein consisting of 244 amino acids. Heavily glycosylated\u003cbr\u003e\nDistribution: Expressed on mouse thymocytes and spleen cells. Highly expressed on natural regulatory (Treg) cells\u003cbr\u003e\nFunction: Mediate calcium-independent homophlic cell-cell adhesion\u003cbr\u003e\nLigand\/Receptor: Folic acid and its derivative\u003cbr\u003e\nCell Type: Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nAntigen References: 1. Yamaguchi T et al 2007. Immunity 27:145 2. Spiegelstein O et al. 2000 Gene 258:117\u003cbr\u003e\nGene ID: 64931\u003cbr\u003e\nUniProt: View information about FR4 on UniProt.org\u003cbr\u003e\nClone: 12A5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Folate Receptor 4, FBP, FR δ\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860795642025,"sku":"125009","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-125009","provider":"Iright","version":"1.0","type":"link"}