{"product_id":"biolegend-125208","title":"Biolegend, 125208, PE anti-mouse CD223 (LAG-3) Antibody, 200μg","description":"\u003cp\u003eCD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.\u003cbr\u003e\n200μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Murine CD223-Ig fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA) Workman CJ, et al. 2005. J. Immunol. 174:688. (FC) Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)\u003cbr\u003e\nProduct Citations: Bertrand F, et al. 2017. Nat Commun. 8:2256. PubMed Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed Swan SL, et al. 2023. Front Immunol. 14:1085547. PubMed Pandit M, et al. 2023. Nat Commun. 14:2593. PubMed Balood M, et al. 2022. Nature. 611:405. PubMed Wei M, et al. 2022. Front Immunol. 13:1017574. PubMed Cheng H, et al. 2023. Nat Metab. 5:314. PubMed Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed Tian H, et al. 2022. Cancer Sci. 113:875. PubMed Lu SX, et al. 2021. Cell. . PubMed Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed Wagle MV, et al. 2021. Nat Commun. 12:2782. PubMed Gozgit JM, et al. 2021. Cancer Cell. :. PubMed Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed Fortner K, et al. 2017. Journal of Autoimmunity. 10.1016\/j.jaut.2017.05.003. PubMed Zuo S, et al. 2021. J Immunother Cancer. 9:. PubMed Shen M, et al. 2022. Nat Cancer. 3:60. PubMed RY H, et al. 2016. Oncoimmunology. 6:e1249561. PubMed Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed Wedekind MF, et al. 2021. iScience. 24(7):102759. PubMed Alenazy MF, et al. 2022. PLoS One. 17:e0271689. PubMed De Souza ALS, et al. 2018. Neurol Ther. 7:103. PubMed Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed Katsuyama T, et al. 2021. Cell Reports. 36(1):109339. PubMed Rudd CE, et al. 2020. Cell Reports. 30(7):2075-2082. PubMed\u003cbr\u003e\nRRID: AB_2133344 (BioLegend Cat. No. 125207) AB_2133343 (BioLegend Cat. No. 125208)\u003cbr\u003e\nStructure: Ig superfamily\u003cbr\u003e\nDistribution: T cells and NK cells, as well as T regulatory cells\u003cbr\u003e\nCell Type: NK cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Immunology, Inhibitory Molecules\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Baixeras E et al. 1992. J. Exp. Med. 176:327.\u003cbr\u003e\nGene ID: 16768\u003cbr\u003e\nUniProt: View information about CD223 on UniProt.org\u003cbr\u003e\nClone: C9B7W\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: LAG3, LAG-3\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862954528937,"sku":"125208","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-125208","provider":"Iright","version":"1.0","type":"link"}