{"product_id":"biolegend-125212","title":"Biolegend, 125212, PerCP\/Cyanine5.5 anti-mouse CD223 (LAG-3) Antibody, 100μg","description":"\u003cp\u003eCD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Murine CD223-Ig fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA) Workman CJ, et al. 2005. J. Immunol. 174:688. (FC) Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)\u003cbr\u003e\nProduct Citations: Jayachandran R, et al. 2019. Immunity. 50:152. PubMed Nguyen T, et al. 2023. Nat Commun. 14:3357. PubMed Chakraborty P, et al. 2022. Cancer Res. 82:1969. PubMed Balood M, et al. 2022. Nature. 611:405. PubMed Liu M, et al. 2022. Cells. 11: . PubMed Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed Zander R, et al. 2022. Immunity. 55:475. PubMed Ertl Y 2014. J Immunol. 193:1836. PubMed Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed Fumagalli V, et al. 2020. J Exp Med. :217. PubMed Vardhana SA, et al. 2020. Nat Immunol. 1.584722222. PubMed Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed Araujo Furlan CL, et al. 2018. Front Immunol. 2.149305556. PubMed Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed Rousso-Noori L, et al. 2021. Nat Commun. 12:3615. PubMed\u003cbr\u003e\nRRID: AB_2561516 (BioLegend Cat. No. 125211) AB_2561517 (BioLegend Cat. No. 125212)\u003cbr\u003e\nStructure: Ig superfamily\u003cbr\u003e\nDistribution: T cells and NK cells, as well as T regulatory cells\u003cbr\u003e\nCell Type: NK cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Immunology, Inhibitory Molecules\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Baixeras E et al. 1992. J. Exp. Med. 176:327.\u003cbr\u003e\nGene ID: 16768\u003cbr\u003e\nUniProt: View information about CD223 on UniProt.org\u003cbr\u003e\nClone: C9B7W\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: LAG3, LAG-3\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862958526633,"sku":"125212","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-125212","provider":"Iright","version":"1.0","type":"link"}