{"product_id":"biolegend-127010","title":"Biolegend, 127010, PE anti-mouse CD317 (BST2, PDCA-1) Antibody, 100μg","description":"\u003cp\u003eCD317, known as BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, or PDCA-1, is type II transmembrane glycoprotein with a molecular mass of 29-33 kD. It is predominantly expressed on Type I IFN-producing cells (IPCs) in naïve mice, but is up-regulated on most cell types following stimulation with type I IFNs and IFN-gamma. It is highly expressed on terminally differentiated normal plasmacytoid dendritic cells and some tumor cells, such as multiple myeloma, renal cell carcinoma, and melanoma cells. BST2 is a recently identified, IFN-induced cellular response factor that blocks release of HIV-1 and other retroviruses from infected cells. BST2 has been found to be the natural ligand of ILT7 in human model.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse plasmacytoid dendritic cells (DCs)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy, functional assay2, and depletion3,4. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt;0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Blasius AL, et al. 2006. J. Immunol. 177:3260. Schliemann C, et al. 2010. Blood 115:736. (FA, IF) Rajagopal D, et al. 2010. Blood 115:1949. (Depletion) Moniz RJ, et al. 2010. FEMS Immunol. Med. Microbiol. 58:397. (Depletion) Chen YL, et al. 2013. J Exp Med. 210:2515. PubMed\u003cbr\u003e\nProduct Citations: Gardet A, et al. 2019. Front Immunol. 275:10. PubMed Chang CA, et al. 2022. Cell Rep. 111615:41. PubMed Kunimura K, et al. 2020. Cell Reports. 29(9):2823-2834.e7.. PubMed de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed Toivonen R, et al. 2016. J Immunol. 196: 4750 - 4759. PubMed Kimura T, et al. 2020. Commun Biol. 0.527777778. PubMed Uddback I, et al. 2016. Sci Rep. 6:20137. PubMed Wanner-Seleznik GM, et al. 2020. Immunohorizons. 0.644444444. PubMed Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed Zegarra‐Ruiz DF et al. 2018. Cell host \u0026amp; microbe. 25(1):113-127 . PubMed Lee A, et al. 2022. Nat Commun. 13:549. PubMed\u003cbr\u003e\nRRID: AB_1953284 (BioLegend Cat. No. 127009) AB_1953285 (BioLegend Cat. No. 127010)\u003cbr\u003e\nStructure: Type II transmembrane glycoprotein with a molecular mass of 29-33 kD.\u003cbr\u003e\nDistribution: Expressed on type I IFN-producing cells, plasmacytoid dendritic cells, and neoplastic B cells, such as multiple myeloma.\u003cbr\u003e\nFunction: Recently identified antiviral protein that blocks the release of nascent retrovirus or other particles from infected cells.\u003cbr\u003e\nCell Type: Dendritic cells\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Douglas JL. et al. 2009. J Virol. 83(16):7931 2. Cao W et al. 2009. J. Exp. Med. 206(7):1603 3. Neil SJ. et al. 2008. Nature 451:425\u003cbr\u003e\nGene ID: 69550\u003cbr\u003e\nUniProt: View information about CD317 on UniProt.org\u003cbr\u003e\nClone: 927\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, PDCA-1\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862500102313,"sku":"127010","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-127010","provider":"Iright","version":"1.0","type":"link"}