{"product_id":"biolegend-127213","title":"Biolegend, 127213, PerCP\/Cyanine5.5 anti-mouse CD73 Antibody, 25μg","description":"\u003cp\u003eCD73 (ecto-5'-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b + myeloid cells. In spleen, it is largely expressed on T cells.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: CHO cells transfected with mouse CD73\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981. Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-P)\u003cbr\u003e\nProduct Citations: Ogishi M, et al. 2023. J Exp Med. 220: . PubMed Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed Dumas SJ, et al. 2021. STAR Protocols. 2(3):100523. PubMed Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed Chen ELY, et al. 2021. Cell Rep. 35:109227. PubMed Duan L, et al. 2021. Immunity. 54:2256. PubMed Mintz MA, et al. 2019. Immunity. 51:310. PubMed Dumas SJ, et al. 2020. J Am Soc Nephrol. 31:118. PubMed Wei C, et al. 2016. Cell Death Dis. 7:e2489. PubMed Zhou J, et al. 2019. Nat Commun. 10:2427. PubMed Raso F, et al. 2018. J Clin Invest. 128:4163. PubMed Young JS, et al. 2018. Front Immunol. 9:1385. PubMed Ott M, et al. 2020. JCI Insight. 5(17):134386. PubMed\u003cbr\u003e\nRRID: AB_11219608 (BioLegend Cat. No. 127213) AB_11219403 (BioLegend Cat. No. 127214)\u003cbr\u003e\nStructure: 69 kD GPI-anchored surface protein\u003cbr\u003e\nDistribution: Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73\u003cbr\u003e\nFunction: A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates\u003cbr\u003e\nLigand\/Receptor: Nucleoside 5' monophosphate\u003cbr\u003e\nCell Type: B cells, Mesenchymal Stem Cells, Tregs\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981. 2. Resta R, et al. 1998. Immunol. Rev. 161:95.\u003cbr\u003e\nRegulation: Expression is regulated by the development and maturation of leukocytes\u003cbr\u003e\nGene ID: 23959\u003cbr\u003e\nUniProt: View information about CD73 on UniProt.org\u003cbr\u003e\nClone: TY\/11.8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Ecto-5'-nucleotidase\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864062120105,"sku":"127213","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-127213","provider":"Iright","version":"1.0","type":"link"}