{"product_id":"biolegend-127216","title":"Biolegend, 127216, Brilliant Violet 605™ anti-mouse CD73 Antibody, 50μg","description":"\u003cp\u003eCD73 (ecto-5'-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b + myeloid cells. In spleen, it is largely expressed on T cells.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: CHO cells transfected with mouse CD73\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610\/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981. Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-P)\u003cbr\u003e\nProduct Citations: Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed Andrade MS, et al. 2022. JCI Insight. 7:. PubMed Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed Perrot I et al. 2019. Cell Rep. 27(8):2411-2425 . PubMed Suah AN, et al. 2021. J Clin Invest. 131:. PubMed Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed Campbell C et al. 2018. Immunity. 48(6):1245-1257 . PubMed Abbott RK, et al. 2018. Immunity. 48:133. PubMed Quiroz HJ, et al. 2022. Front Cardiovasc Med. 8:826687. PubMed Watson MJ, et al. 2021. Nature. 591:645. PubMed Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed Jones D, et al. 2015. J Immunol. 195: 4753 - 4759. PubMed Shen H, et al. 2022. Nat Commun. 13:5013. PubMed\u003cbr\u003e\nRRID: AB_2561528 (BioLegend Cat. No. 127215)\u003cbr\u003e\nStructure: 69 kD GPI-anchored surface protein\u003cbr\u003e\nDistribution: Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73\u003cbr\u003e\nFunction: A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates\u003cbr\u003e\nLigand\/Receptor: Nucleoside 5' monophosphate\u003cbr\u003e\nCell Type: B cells, Mesenchymal Stem Cells, Tregs\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981. 2. Resta R, et al. 1998. Immunol. Rev. 161:95.\u003cbr\u003e\nRegulation: Expression is regulated by the development and maturation of leukocytes\u003cbr\u003e\nGene ID: 23959\u003cbr\u003e\nUniProt: View information about CD73 on UniProt.org\u003cbr\u003e\nClone: TY\/11.8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Ecto-5'-nucleotidase\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864062349481,"sku":"127216","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-127216","provider":"Iright","version":"1.0","type":"link"}